Skip to main content
PMC home page
Genetics logoLink to Genetics
. 1981 Aug;98(4):677–689. doi: 10.1093/genetics/98.4.677

High Frequency of Genetic Duplications in the dnaB Region of the ESCHERICHIA COLI K12 Chromosome

Robert A Sclafani 1,2, James A Wechsler 1,2
PMCID: PMC1214468  PMID: 7037536

Abstract

The region that includes the dnaB locus on the E. coli K12 chromosome was shown to be duplicated at high frequency in cell populations. The duplications were shown to be arranged in tandem and segregated at various frequencies. Segregation was dependent on the recA recombination system, but independent of recB,C. Though most of the data was obtained with dnaB::Tn10 insertion mutants, the duplications were shown to occur in the absence of Tn10.

Full Text

The Full Text of this article is available as a PDF (772.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. APPLEYARD R. K., MCGREGOR J. F., BAIRD K. M. Mutation to extended host range and the occurrence of phenotypic mixing in the temperate coliphage lambda. Virology. 1956 Aug;2(4):565–574. doi: 10.1016/0042-6822(56)90012-5. [DOI] [PubMed] [Google Scholar]
  2. Ames B. N., Durston W. E., Yamasaki E., Lee F. D. Carcinogens are mutagens: a simple test system combining liver homogenates for activation and bacteria for detection. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2281–2285. doi: 10.1073/pnas.70.8.2281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Anderson R. P., Roth J. R. Tandem genetic duplications in phage and bacteria. Annu Rev Microbiol. 1977;31:473–505. doi: 10.1146/annurev.mi.31.100177.002353. [DOI] [PubMed] [Google Scholar]
  4. Bachmann B. J., Low K. B. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. doi: 10.1128/mr.44.1.1-56.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Barclay B. J., Little J. G. Genetic damage during thymidylate starvation in Saccharomyces cerevisiae. Mol Gen Genet. 1978 Mar 20;160(1):33–40. doi: 10.1007/BF00275116. [DOI] [PubMed] [Google Scholar]
  7. Cox B. S., Tuite M. F., Mundy C. J. Reversion from suppression to nonsuppression in SUQ5 [psi+] strains of yeast: the classificaion of mutations. Genetics. 1980 Jul;95(3):589–609. doi: 10.1093/genetics/95.3.589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Culbertson M. R., Charnas L., Johnson M. T., Fink G. R. Frameshifts and frameshift suppressors in Saccharomyces cerevisiae. Genetics. 1977 Aug;86(4):745–764. doi: 10.1093/genetics/86.4.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. EPHRUSSI B., HOTTINGUER H. On an unstable cell state in yeast. Cold Spring Harb Symp Quant Biol. 1951;16:75–85. doi: 10.1101/sqb.1951.016.01.007. [DOI] [PubMed] [Google Scholar]
  10. Evans W. E., Parry J. M. The genetic effects of elevated temperature in the yeast, Saccharomyces cerevisiae. Heredity (Edinb) 1975 Dec;35(3):347–359. doi: 10.1038/hdy.1975.105. [DOI] [PubMed] [Google Scholar]
  11. Fink G. R., Styles C. A. Curing of a killer factor in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2846–2849. doi: 10.1073/pnas.69.10.2846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Friend C., Freedman H. A. Effects and possible mechanism of action of dimethylsulfoxide on Friend cell differentiation. Biochem Pharmacol. 1978 May 1;27(9):1309–1313. doi: 10.1016/0006-2952(78)90112-0. [DOI] [PubMed] [Google Scholar]
  13. Hopwood D. A. Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriol Rev. 1967 Dec;31(4):373–403. doi: 10.1128/br.31.4.373-403.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Juliani M. H., Gambarini A. G., Costa S. O. Induction of rho-minus mutants in Saccharomyces cerevisiae by guanidine hydrochloride. I. Genetic analysis. Mutat Res. 1975 Jul;29(1):67–75. [PubMed] [Google Scholar]
  15. LEVINE L., GORDON J. A., JENCKS W. P. The relationship of structure to the effectiveness of denaturing agents for deoxyribonucleic acid. Biochemistry. 1963 Jan-Feb;2:168–175. doi: 10.1021/bi00901a030. [DOI] [PubMed] [Google Scholar]
  16. Lark K. G., Wechsler J. A. DNA replication in dnaB mutants of Escherichia coli: gene product interaction and synthesis of 4 S pieces. J Mol Biol. 1975 Feb 15;92(1):145–163. doi: 10.1016/0022-2836(75)90095-9. [DOI] [PubMed] [Google Scholar]
  17. McCready S. J., Cox B. S. Antisuppressors in yeast. Mol Gen Genet. 1973 Aug 28;124(4):305–320. doi: 10.1007/BF00267660. [DOI] [PubMed] [Google Scholar]
  18. NAGAI S., YANAGISHIMA N., NAGAI H. Advances in the study of respiration-deficient (RD) mutation in yeast and other microorganisms. Bacteriol Rev. 1961 Dec;25:404–426. doi: 10.1128/br.25.4.404-426.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nakanishi S., Adhya S., Gottesman M., Pastan I. Effects of dimethylsulfoxide on the E. coli gal operon and on bacteriophage lambda in vivo. Cell. 1974 Sep;3(1):39–46. doi: 10.1016/0092-8674(74)90035-x. [DOI] [PubMed] [Google Scholar]
  20. Newlon C. S., Ludescher R. D., Walter S. K. Production of petites by cell cycle mutants of Saccharomyces cerevisiae defective in DNA synthesis. Mol Gen Genet. 1979 Jan 31;169(2):189–194. doi: 10.1007/BF00271670. [DOI] [PubMed] [Google Scholar]
  21. Oliver S. G., Williamson D. H. The molecular events involved in the induction of petite yeast mutants by fluorinated pyrimidines. Mol Gen Genet. 1976 Aug 2;146(3):253–259. doi: 10.1007/BF00701248. [DOI] [PubMed] [Google Scholar]
  22. Ono B. I., Stewart J. W., Sherman F. Yeast UAA suppressors effective in psi+ strains serine-inserting suppressors. J Mol Biol. 1979 Feb 15;128(1):81–100. doi: 10.1016/0022-2836(79)90309-7. [DOI] [PubMed] [Google Scholar]
  23. Porter R. D., McLaughlin T., Low B. Transduction versus "conjuduction": evidence for multiple roles for exonuclease V in genetic recombination in Escherichia coli. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):1043–1047. doi: 10.1101/sqb.1979.043.01.113. [DOI] [PubMed] [Google Scholar]
  24. Putrament A., Baranowska H., Prazmo W. Induction by manganese of mitochondrial antibiotic resistance mutations in yeast. Mol Gen Genet. 1973 Nov 22;126(4):357–366. doi: 10.1007/BF00269445. [DOI] [PubMed] [Google Scholar]
  25. Sclafani R. A., Wechsler J. A., Schuster H. The isolation and characterization of escherichia coli dnaB::Tn10 insertion mutations. Mol Gen Genet. 1981;182(1):112–118. doi: 10.1007/BF00422776. [DOI] [PubMed] [Google Scholar]
  26. Singh A., Helms C., Sherman F. Mutation of the non-Mendelian suppressor, Psi, in yeast by hypertonic media. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1952–1956. doi: 10.1073/pnas.76.4.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Singh D. R., Mahajan J. M., Krishnan D. Effect of dimethyl sulfoxide (DMSO) on radiation-induced heteroallelic reversion in diploid yeast. Mutat Res. 1976 Nov;37(2-3):193–200. doi: 10.1016/0027-5107(76)90033-6. [DOI] [PubMed] [Google Scholar]
  28. Slonimski P. P., Perrodin G., Croft J. H. Ethidium bromide induced mutation of yeast mitochondria: complete transformation of cells into respiratory deficient non-chromosomal "petites". Biochem Biophys Res Commun. 1968 Feb 15;30(3):232–239. doi: 10.1016/0006-291x(68)90440-3. [DOI] [PubMed] [Google Scholar]
  29. Soll L., Berg P. Recessive lethals: a new class of nonsense suppressors in Escherichia coli. Proc Natl Acad Sci U S A. 1969 Jun;63(2):392–399. doi: 10.1073/pnas.63.2.392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Touati-Schwartz D. A dnaB analog ban, specified by bacteriophage P1: genetic and physiological evidence for functional analogy and interactions between the two products. Mol Gen Genet. 1979 Jul 13;174(2):173–188. doi: 10.1007/BF00268354. [DOI] [PubMed] [Google Scholar]
  31. Wickner R. B. "Killer character" of Saccharomyces cerevisiae: curing by growth at elevated temperature. J Bacteriol. 1974 Mar;117(3):1356–1357. doi: 10.1128/jb.117.3.1356-1357.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Williamson D. H., Maroudas N. G., Wilkie D. Induction of the cytoplasmic petite mutation in Saccharomyces cerevisiae by the antibacterial antibiotics erythromycin and chloramphenicol. Mol Gen Genet. 1971;111(3):209–223. doi: 10.1007/BF00433106. [DOI] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES