Abstract
Forty-eight new mutations at the am locus of Neurospora crassa have been characterized. Nineteen mutations were induced by UV; of these, eight were missense, two were frameshifts, two were nonsense, three were deletions and four were unidentified. Twenty-nine mutations were induced with nitrous acid; of these, twenty-one were missense, three were frameshifts, one was nonsense, two were deletions and one was genetically unstable.
Full Text
The Full Text of this article is available as a PDF (625.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Alper M. D., Ames B. N. Positive selection of mutants with deletions of the gal-chl region of the Salmonella chromosome as a screening procedure for mutagens that cause deletions. J Bacteriol. 1975 Jan;121(1):259–266. doi: 10.1128/jb.121.1.259-266.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coddington A., Fincham J. R., Sundaram T. K. Multiple active varieties of Neurospora glutamate dehydrogenase formed by hybridization between two inactive mutant proteins in vivo and in vitro. J Mol Biol. 1966 Jun;17(2):503–512. doi: 10.1016/s0022-2836(66)80160-2. [DOI] [PubMed] [Google Scholar]
- Culbertson M. R., Charnas L., Johnson M. T., Fink G. R. Frameshifts and frameshift suppressors in Saccharomyces cerevisiae. Genetics. 1977 Aug;86(4):745–764. doi: 10.1093/genetics/86.4.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fincham J. R., Baron A. J. The molecular basis of an osmotically reparable mutant of Neurospora crassa producing unstable glutamate dehydrogenase. J Mol Biol. 1977 Mar 15;110(4):627–642. doi: 10.1016/s0022-2836(77)80081-8. [DOI] [PubMed] [Google Scholar]
- Kinsey J. A. Direct selective procedure for isolating Neurospora mutants defective in nicotinamide adenine dinucleotide phosphate-specific glutamate dehydrogenase. J Bacteriol. 1977 Dec;132(3):751–756. doi: 10.1128/jb.132.3.751-756.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smyth D. R. A new map of the amination-1 locus of Neurospora crassa, and the effect of the recombination-3 gene. Aust J Biol Sci. 1973 Dec;26(6):1355–1370. doi: 10.1071/bi9731355. [DOI] [PubMed] [Google Scholar]
- Stadler D. R. Glutamic dehydrogenase in revertants of am mutants in Neurospora. Genet Res. 1966 Feb;7(1):18–31. doi: 10.1017/s0016672300009447. [DOI] [PubMed] [Google Scholar]
- Wootton J. C., Chambers G. K., Holder A. A., Baron A. J., Taylor J. G., Fincham J. R., Blumenthal K. M., Moon K., Smith E. L. Amino-acid sequence of NADP-specific glutamate dehydrogenase of neurospora crassa. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4361–4365. doi: 10.1073/pnas.71.11.4361. [DOI] [PMC free article] [PubMed] [Google Scholar]