Abstract
1. A simple method for the extraction of 17-oxo steroid sulphates of plasma is described; glucosiduronates and orthophosphates are extracted, but to a smaller extent. 2. Four methods of analyses of the extracts are given and are relatively simple. Three of these are specific for steroid sulphates and two measure the sulphate conjugates directly. 3. Values for dehydroepiandrosterone sulphate and androsterone sulphate concentrations of normal and pathological plasmas are given.
Full text
PDF










Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BAULIEU E. E. Studies of conjugated 17-ketosteroids in a case of adrenal tumor. J Clin Endocrinol Metab. 1962 May;22:501–510. doi: 10.1210/jcem-22-5-501. [DOI] [PubMed] [Google Scholar]
- BAULIEU E. E. Three sulfate esters of 17-ketosteroids in the plasma of normal subjects and after administration of ACTH. J Clin Endocrinol Metab. 1960 Jun;20:900–904. doi: 10.1210/jcem-20-6-900. [DOI] [PubMed] [Google Scholar]
- BONDY P. K., COHN G. L., HERRMANN W., CRISPELL K. R. The possible relationship of etiocholanolone to periodic fever. Yale J Biol Med. 1958 Jun;30(6):395–405. [PMC free article] [PubMed] [Google Scholar]
- BROOKS R. V. A method for the quantitative fractionation of urinary 17-oxo steroids with some observations on steroid excretion during administration of ACTH and in the adrenogenital syndrome. Biochem J. 1958 Jan;68(1):50–57. doi: 10.1042/bj0680050. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BUSH I. E. Methods of paper chromatography of steroids applicable to the study of steroids in mammalian blood and tissues. Biochem J. 1952 Jan;50(3):370–378. doi: 10.1042/bj0500370. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COHEN S. L., ONESON I. B. The conjugated steroids. IV. The hydrolysis of ketosteroid sulfates. J Biol Chem. 1953 Sep;204(1):245–256. [PubMed] [Google Scholar]
- COHN G. L., BONDY P. K., CASTIGLIONE C. Studies on pyrogenic steroids. I. Separation, identification, and measurement of unconjugated dehydroepiandrosterone, etiocholanolone, and adrosterone in human plasma. J Clin Invest. 1961 Feb;40:400–407. doi: 10.1172/JCI104267. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CONRAD S., MAHESH V., HERRMANN W. Quantitative paper chromatography of conjugated 17-ketosteroids in plasma. J Clin Invest. 1961 Jun;40:947–953. doi: 10.1172/JCI104334. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CORKER C. S., NORYMBERSKI J. K., THOW R. Some aspects of the Zimmermann reaction. Biochem J. 1962 Jun;83:583–588. doi: 10.1042/bj0830583. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CREPY O., JUDAS O. [Quantitative and qualitative determination of steroid sulfates with the aid of methylene blue. II. Detection of steroid sulfates by paper chromatography]. Rev Fr Etud Clin Biol. 1960 Mar;5:284–286. [PubMed] [Google Scholar]
- FOTHERBY K. The isolation of 3 beta-hydroxy-delta 5-steroids from the urine of normal men. Biochem J. 1958 Aug;69(4):596–600. doi: 10.1042/bj0690596. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KELLIE A. E., SMITH E. R. Renal clearance of 17-oxo steroid conjugates found in human peripheral plasma. Biochem J. 1957 Jul;66(3):490–495. doi: 10.1042/bj0660490. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MAHESH V. B., GREENBLATT R. B. Isolation of dehydroepiandrosterone and 17alpha-hydroxy-delta5-pregenolone from the polycystic ovaries of the Stein-Leventhal syndrome. J Clin Endocrinol Metab. 1962 Apr;22:441–448. doi: 10.1210/jcem-22-4-441. [DOI] [PubMed] [Google Scholar]
- MIGEON C. J., TYLER F. H., MAHONEY J. P., FLORENTIN A. A., CASTLE H., BLISS E. L., SAMUELS L. T. The diurnal variation of plasma levels and urinary excretion on 17-hydroxycorticosteroids in normal subjects, night workers and blind subjects. J Clin Endocrinol Metab. 1956 May;16(5):622–633. doi: 10.1210/jcem-16-5-622. [DOI] [PubMed] [Google Scholar]
- Menini E., Norymberski J. K. The oxidation of steroids with tert.-butyl chromate. Biochem J. 1962 Jul;84(1):195–201. doi: 10.1042/bj0840195. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OERTEL G. W., EIK-NES K. B. Isolation and identification of etiocholan-3 alpha-ol-17-one and etiocholane-3 alpha, 11 beta-diol-17-one from human blood plasma. Arch Biochem Biophys. 1961 Jan;92:150–153. doi: 10.1016/0003-9861(61)90229-6. [DOI] [PubMed] [Google Scholar]
- OERTEL G. W., KAISER E. Determination of dehydroepian-drosterone, androsterone, etiocholanolone, and "11 beta-hydroxyetiocholanolone" in plasma. Clin Chim Acta. 1962 Mar;7:221–225. doi: 10.1016/0009-8981(62)90013-x. [DOI] [PubMed] [Google Scholar]
- ROY A. B. The steroid sulphatase of Patella vulgata. Biochem J. 1956 Jan;62(1):41–50. doi: 10.1042/bj0620041. [DOI] [PMC free article] [PubMed] [Google Scholar]