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. 1965 Apr;95(1):161–166. doi: 10.1042/bj0950161

The estimation of the oxidized and reduced forms of the nicotinamide nucleotides

A L Greenbaum 1,2, J B Clark 1,2, Patricia McLean 1,2
PMCID: PMC1215190  PMID: 14333552

Abstract

1. A method is described for the determination of the oxidized and reduced forms of the nicotinamide nucleotides by measuring the rate of the oxygen uptake with an oxygen electrode in a system in which the nucleotide acts as the rate-limiting carrier in a cyclic system. 2. The method permits the measurement of quantities as low as 0·02μg. of NAD+ or NADH or 0·01μg. of NADP+ or NADPH. 3. The method permits the measurement of the nucleotides in extracts that contain non-specific reducing substances, coloured compounds or fluorescent materials, e.g. green leaves. 4. The results obtained by the present method are compared with those reported in the literature.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSON D. G., VENNESLAND B. The occurrence of di- and triphosphopyridine nucleotides in green leaves. J Biol Chem. 1954 Apr;207(2):613–620. [PubMed] [Google Scholar]
  2. BASSHAM J. A., BIRT L. M., HEMS R., LOENING U. E. Determination of the reduced and oxidized pyridine nucleotides in animal tissues. Biochem J. 1959 Nov;73:491–499. doi: 10.1042/bj0730491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BRIGGS M. H. Vitamin and coenzyme content of hepatomas induced by buter yellow. Nature. 1960 Jul 16;187:249–250. doi: 10.1038/187249a0. [DOI] [PubMed] [Google Scholar]
  4. CAIGER P., MORTON R. K., FILSELL O. H., JARRETT I. G. A comparative study of nicotinamide nucleotide coenzymes during growth of the sheep and rat. Biochem J. 1962 Nov;85:351–359. doi: 10.1042/bj0850351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CHANCE B., SCHOENER B. Correlation of oxidation-reduction changes of intracellular reduced pyridine nucleotide and changes in electroencephalogram of the rat in anoxia. Nature. 1962 Sep 8;195:956–958. doi: 10.1038/195956a0. [DOI] [PubMed] [Google Scholar]
  6. Dickens F., McIlwain H. Phenazine compounds as carriers in the hexosemonophosphate system. Biochem J. 1938 Sep;32(9):1615–1625. doi: 10.1042/bj0321615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GLOCK G. E., MCLEAN P. Effects of hormones on levels of oxidized and reduced diphosphopyridine nucleotide and triphosphopyridine nucleotide in liver and diaphragm. Biochem J. 1955 Nov;61(3):397–402. doi: 10.1042/bj0610397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GLOCK G. E., MCLEAN P. Levels of oxidized and reduced diphosphopyridine nucleotide and triphosphopyridine nucleotide in tumours. Biochem J. 1957 Feb;65(2):413–416. doi: 10.1042/bj0650413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GLOCK G. E., MCLEAN P. The determination of oxidized and reduced diphosphopyridine nucleotide and triphosphopyridine nucleotide in animal tissues. Biochem J. 1955 Nov;61(3):381–388. doi: 10.1042/bj0610381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HOHORST H. J., KREUTZ F. H., REIM M. Steady state equilibria of some DPN-linked reactions and the oxidation/reduction state of the DPN/DPNH system in the cytoplasmatic compartment of liver cells in vivo. Biochem Biophys Res Commun. 1961 Mar 10;4:159–162. doi: 10.1016/0006-291x(61)90262-5. [DOI] [PubMed] [Google Scholar]
  11. JACOBSON K. B., ASTRACHAN L. Specific methods for the microdetermination of pyridine nucleotides and pyridine nucleotide enzymes. Arch Biochem Biophys. 1957 Sep;71(1):69–80. doi: 10.1016/0003-9861(57)90008-5. [DOI] [PubMed] [Google Scholar]
  12. JACOBSON K. B., KAPLAN N. O. Pyridine coenzymes of subcellular tissue fractions. J Biol Chem. 1957 Jun;226(2):603–613. [PubMed] [Google Scholar]
  13. LOWRY O. H., PASSONNEAU J. V., SCHULZ D. W., ROCK M. K. The measurement of pyridine nucleotides by enzymatic cycling. J Biol Chem. 1961 Oct;236:2746–2755. [PubMed] [Google Scholar]
  14. McLEAN P. Carbohydrate metabolism of mammary tissue. II. Levels of oxidised and reduced diphosphopyridine nucleotide and triphosphopyridine nucleotide in the rat mammary gland. Biochim Biophys Acta. 1958 Nov;30(2):316–324. doi: 10.1016/0006-3002(58)90056-8. [DOI] [PubMed] [Google Scholar]
  15. NODES J. T., REID E. AZO-DYE CARCINOGENESIS: RIBONUCLEOTIDES AND RIBONUCLEASES. Br J Cancer. 1963 Dec;17:745–774. doi: 10.1038/bjc.1963.96. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. PASTAN I., WILLS V., HERRING B., FIELD J. B. PYRIDINE NUCLEOTIDES IN THE THYROID. I. A METHOD FOR THE MEASUREMENT OF OXIDIZED AND REDUCED TRIPHOSPHOPYRIDINE NUCLEOTIDES WITH THE USE OF 6-PHOSPHOGLUCONATE-1-C14. J Biol Chem. 1963 Oct;238:3362–3365. [PubMed] [Google Scholar]
  17. SLATER T. F., SAWYER B. A colorimetric method for estimating the pyridine nucleotide content of small amounts of animal tissue. Nature. 1962 Feb 3;193:454–456. doi: 10.1038/193454a0. [DOI] [PubMed] [Google Scholar]
  18. Slater T. F., Sawyer B., Sträuli U. An assay procedure for nicotinamide-adenine dinucleotides in rat liver and other tissues. Arch Int Physiol Biochim. 1964 Jun;72(3):427–447. doi: 10.3109/13813456409065351. [DOI] [PubMed] [Google Scholar]
  19. VILLEE C. A. An enzymic method for the assay of pyridine nucleotides in extracts of animal tissues. Biochem J. 1962 Apr;83:191–194. doi: 10.1042/bj0830191. [DOI] [PMC free article] [PubMed] [Google Scholar]

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