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. 1985 Mar;109(3):513–528. doi: 10.1093/genetics/109.3.513

Genetic Mapping of CAENORHABDITIS ELEGANS Collagen Genes Using DNA Polymorphisms as Phenotypic Markers

George N Cox 1, Stephen Carr 1, James M Kramer 1, David Hirsh 1
PMCID: PMC1216285  PMID: 3979813

Abstract

In Caenorhabditis elegans collagens comprise a dispersed family of 40–150 genes, the majority of which probably code for collagen proteins found in the animal's cuticle. The conserved (Gly-X-Y)n triple helix coding sequence of collagen genes has facilitated the isolation of a large number of C. elegans collagen genes by recombinant DNA methods. We have begun a study of the chromosomal organization of these genes by screening laboratory strains of C. elegans for DNA polymorphisms in the regions surrounding collagen genes. Polymorphisms near seven genes have been identified and have been used as phenotypic markers in genetic crosses to assign the genes to linkage groups II, III, IV, and X. Four genes are shown by multifactor crosses to map to a 2–3 map unit interval between unc-24 and unc-22 on chromosome IV.

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Selected References

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  1. Bornstein P., Sage H. Structurally distinct collagen types. Annu Rev Biochem. 1980;49:957–1003. doi: 10.1146/annurev.bi.49.070180.004521. [DOI] [PubMed] [Google Scholar]
  2. Clewell D. B. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol. J Bacteriol. 1972 May;110(2):667–676. doi: 10.1128/jb.110.2.667-676.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cox G. N., Kramer J. M., Hirsh D. Number and organization of collagen genes in Caenorhabditis elegans. Mol Cell Biol. 1984 Nov;4(11):2389–2395. doi: 10.1128/mcb.4.11.2389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cox G. N., Laufer J. S., Kusch M., Edgar R. S. Genetic and Phenotypic Characterization of Roller Mutants of CAENORHABDITIS ELEGANS. Genetics. 1980 Jun;95(2):317–339. doi: 10.1093/genetics/95.2.317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Davies J., Nomura M. The genetics of bacterial ribosomes. Annu Rev Genet. 1972;6:203–234. doi: 10.1146/annurev.ge.06.120172.001223. [DOI] [PubMed] [Google Scholar]
  6. Emmons S. W., Yesner L., Ruan K. S., Katzenberg D. Evidence for a transposon in Caenorhabditis elegans. Cell. 1983 Jan;32(1):55–65. doi: 10.1016/0092-8674(83)90496-8. [DOI] [PubMed] [Google Scholar]
  7. Goldsmith M. R., Basehoar G. Organization of the chorion genes of Bombyx mori, a multigene family. I. Evidence for linkage to chromosome 2. Genetics. 1978 Oct;90(2):291–310. doi: 10.1093/genetics/90.2.291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kedes L. H. Histone genes and histone messengers. Annu Rev Biochem. 1979;48:837–870. doi: 10.1146/annurev.bi.48.070179.004201. [DOI] [PubMed] [Google Scholar]
  9. Kramer J. M., Cox G. N., Hirsh D. Comparisons of the complete sequences of two collagen genes from Caenorhabditis elegans. Cell. 1982 Sep;30(2):599–606. doi: 10.1016/0092-8674(82)90256-2. [DOI] [PubMed] [Google Scholar]
  10. Liao L. W., Rosenzweig B., Hirsh D. Analysis of a transposable element in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3585–3589. doi: 10.1073/pnas.80.12.3585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MacLeod A. R., Waterston R. H., Fishpool R. M., Brenner S. Identification of the structural gene for a myosin heavy-chain in Caenorhabditis elegans. J Mol Biol. 1977 Jul;114(1):133–140. doi: 10.1016/0022-2836(77)90287-x. [DOI] [PubMed] [Google Scholar]
  12. Rosenzweig B., Liao L. W., Hirsh D. Sequence of the C. elegans transposable element Tc1. Nucleic Acids Res. 1983 Jun 25;11(12):4201–4209. doi: 10.1093/nar/11.12.4201. [DOI] [PMC free article] [PubMed] [Google Scholar]

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