Skip to main content
PMC home page
Genetics logoLink to Genetics
. 1987 Mar;115(3):451–460. doi: 10.1093/genetics/115.3.451

Allosuppressors That Enhance the Efficiency of Omnipotent Suppressors in Saccharomyces cerevisiae

Jae Mahn Song 1, Susan W Liebman 1
PMCID: PMC1216348  PMID: 3552876

Abstract

Two recessive Mendelian-allosuppressors have been isolated and have been shown to enhance the efficiency of omnipotent suppressors thought to be translational ambiguity mutations. These allosuppressors are unlinked to each other or to the omnipotent suppressors on which they act. They also increase the efficiency of the serine-inserting UAA-suppressor, SUP16 . One allosuppressor is allelic or tightly linked to the previously isolated sal2. Another allosuppressor, called sal6, represents a new locus, unlinked to the previously isolated sal1-sal5 that enhance the efficiency of the UAA-suppressors. When present singly in the absence of suppressors or other modifiers the sal2 and sal6 mutations do not have suppressor activity. However, when sal2 and sal6 are combined together in a haploid cell they do suppress weakly. In addition sal2 becomes a weak suppressor in the presence of the [η +] modifying factor.

Full Text

The Full Text of this article is available as a PDF (946.3 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkinson K. D. Two recessive suppressors of Saccharomyces cerevisiae cho1 that are unlinked but fall in the same complementation group. Genetics. 1985 Sep;111(1):1–6. doi: 10.1093/genetics/111.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Etcheverry T., Colby D., Guthrie C. A precursor to a minor species of yeast tRNASer contains an intervening sequence. Cell. 1979 Sep;18(1):11–26. doi: 10.1016/0092-8674(79)90349-0. [DOI] [PubMed] [Google Scholar]
  3. Eustice D. C., Wakem L. P., Wilhelm J. M., Sherman F. Altered 40 S ribosomal subunits in omnipotent suppressors of yeast. J Mol Biol. 1986 Mar 20;188(2):207–214. doi: 10.1016/0022-2836(86)90305-0. [DOI] [PubMed] [Google Scholar]
  4. Hawthorne D. C., Leupold U. Suppressors in yeast. Curr Top Microbiol Immunol. 1974;64(0):1–47. doi: 10.1007/978-3-642-65848-8_1. [DOI] [PubMed] [Google Scholar]
  5. Ishiguro J., Ono B. I., Masurekar M., McLaughlin C. S., Sherman F. Altered ribosomal protein S11 from the SUP46 suppressor of yeast. J Mol Biol. 1981 Apr 15;147(3):391–397. doi: 10.1016/0022-2836(81)90491-5. [DOI] [PubMed] [Google Scholar]
  6. Liebman S. W., Cavenagh M. An antisuppressor that acts on omnipotent suppressors in yeast. Genetics. 1980 May;95(1):49–61. doi: 10.1093/genetics/95.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Liebman S. W., Sherman F. Extrachromosomal psi+ determinant suppresses nonsense mutations in yeast. J Bacteriol. 1979 Sep;139(3):1068–1071. doi: 10.1128/jb.139.3.1068-1071.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Liebman S. W., Sherman F., Stewart J. W. Isolation and characterization of amber suppressors in yeast. Genetics. 1976 Feb;82(2):251–272. doi: 10.1093/genetics/82.2.251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Liebman S. W., Srodulski Z., Reed C. R., Stewart J. W., Sherman F., Brennan G. Yeast amber suppressors corresponding to tRNA3Leu genes. J Mol Biol. 1984 Sep 15;178(2):209–226. doi: 10.1016/0022-2836(84)90140-2. [DOI] [PubMed] [Google Scholar]
  10. McCready S. J., Cox B. S. Antisuppressors in yeast. Mol Gen Genet. 1973 Aug 28;124(4):305–320. doi: 10.1007/BF00267660. [DOI] [PubMed] [Google Scholar]
  11. Mortimer R. K., Hawthorne D. C. Genetic mapping in Saccharomyces. Genetics. 1966 Jan;53(1):165–173. doi: 10.1093/genetics/53.1.165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ono B. I., Stewart J. W., Sherman F. Yeast UAA suppressors effective in psi+ strains serine-inserting suppressors. J Mol Biol. 1979 Feb 15;128(1):81–100. doi: 10.1016/0022-2836(79)90309-7. [DOI] [PubMed] [Google Scholar]
  13. Ono B. I., Stewart J. W., Sherman F. Yeast UAA suppressors effective in psi+ strains: leucine-inserting suppressors. J Mol Biol. 1979 Aug 15;132(3):507–520. doi: 10.1016/0022-2836(79)90272-9. [DOI] [PubMed] [Google Scholar]
  14. Randsholt N., Ibarrondo F., Dequard M., Picard-Bennoun M. Analysis of revertants of a ribosomal mutation in Podospora anserina: evidence for new ribosomal mutations which confer hypersensitivity to paromomycin. Biochem Genet. 1982 Jun;20(5-6):569–584. doi: 10.1007/BF00484705. [DOI] [PubMed] [Google Scholar]
  15. Smirnov V. N., Kreier V. G., Lizlova L. V., Andrianova V. M., Inge-Vechtomov S. G. Recessive super-suppression in yeast. Mol Gen Genet. 1974 Mar 14;129(2):105–121. doi: 10.1007/BF00268625. [DOI] [PubMed] [Google Scholar]
  16. Smirnov V. N., Kreier V. G., Lizlova L. V., Inge-Vechtomov S. G. Recessive suppression and protein synthesis in yeast. FEBS Lett. 1973 Dec 15;38(1):96–100. doi: 10.1016/0014-5793(73)80522-8. [DOI] [PubMed] [Google Scholar]
  17. Smirnov V. N., Surguchov A. P., Fominykch E. S., Lizlova L. V., Saprygina T. V., Inge-Vechtomov S. G. Recessive nonsense-suppression in yeast: further characterization of a defect in translation. FEBS Lett. 1976 Jul 1;66(1):12–15. doi: 10.1016/0014-5793(76)80573-x. [DOI] [PubMed] [Google Scholar]
  18. Surguchov A. P., Beretetskaya Y. V., Fominykch E. S., Pospelova E. M., SmirnovVN, Ter-Avanesyan M. D., Inge-Vechtomov S. G. Recessive suppression in yeast Saccharomyces cerevisiae is mediated by a ribosomal mutation. FEBS Lett. 1980 Feb 25;111(1):175–178. doi: 10.1016/0014-5793(80)80786-1. [DOI] [PubMed] [Google Scholar]
  19. Thuriaux P., Minet M., Hofer F., Leupold U. Genetic analysis of antisuppressor mutants in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet. 1976 Dec 31;142(4):251–261. doi: 10.1007/BF00271250. [DOI] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES