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. 1996 Feb 15;314(Pt 1):15–19. doi: 10.1042/bj3140015

Characterization of Saccharomyces cerevisiae deficient in expression of phospholipase D.

K M Ella 1, J W Dolan 1, C Qi 1, K E Meier 1
PMCID: PMC1217018  PMID: 8660276

Abstract

A gene encoding phospholipase D (PLD) in Saccharomyces cerevisiae was identified. The 195 kDa product of PLD1 has 24% overall sequence identity with a plant PLD. Expression of yeast PLD activity was eliminated by one-step gene disruption. Yeast haploids lacking PLD activity were deficient in growth on non-fermentable carbon sources. Diploids lacking expression of PLD1 were unable to sporulate.

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Selected References

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