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. 1996 Aug 1;317(Pt 3):865–870. doi: 10.1042/bj3170865

Calcium-dependent ADP-ribosylation of high-mobility-group I (HMGI) proteins.

V Giancotti 1, A Bandiera 1, C Sindici 1, L Perissin 1, C Crane-Robinson 1
PMCID: PMC1217565  PMID: 8760375

Abstract

Micrococcal nuclease digestion of nuclei from mouse Lewis lung carcinoma cells releases a protein mixture into the supernatant that lacks histone H1 and contains a full complement of high-mobility-group I (HMGI) proteins (i.e. I, Y and I-C). This implies that all three HMGI proteins are localized at the nuclease-sensitive regions of active chromatin. It is also shown that if Ca2+ ions are present in the nuclear incubation buffer (with or without exogenous nuclease), all three HMGI proteins become ADP-ribosylated. We propose that this modification of HMGI family proteins is part of the general poly(ADP-ribosyl)ation that accompanies DNA damage in apoptosis and other processes.

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Selected References

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