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. 1996 Sep 1;318(Pt 2):489–495. doi: 10.1042/bj3180489

Mediation of interleukin-11-dependent biological responses by a soluble form of the interleukin-11 receptor.

J Karow 1, K R Hudson 1, M A Hall 1, A B Vernallis 1, J A Taylor 1, A Gossler 1, J K Heath 1
PMCID: PMC1217647  PMID: 8809037

Abstract

Interleukin-11 (IL-11) is a polyfunctional cytokine whose biological actions require a specific IL-11 receptor (IL-11R) and the transmembrane transducer gp130. Here we report the production of a soluble form of the murine IL-11R and demonstrate that it interacts with IL-11 ligand with high affinity. The affinity of IL-11 alone for gp130 is below the level of detection, but a complex of IL-11 and soluble IL-11R interacts with gp130 with high affinity. The addition of soluble IL-11R potentiates the effects of exogenous IL-11 in cells that are normally responsive to IL-11. A biological response to IL-11 can be reconstituted in BAF cells transfected with gp130 by addition of IL-11 and soluble IL-11R. These findings show that the cytoplasmic domain of the IL-11R is not required for the biological effects of IL-11 and that a complex of IL-11 and IL-11R mediates signalling by association with gp130.

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Selected References

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