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. 1996 Nov 15;320(Pt 1):247–252. doi: 10.1042/bj3200247

Evidence for a novel cytoplasmic tRNA-protein complex containing the KH-multidomain protein vigilin.

C Kruse 1, A Grünweller 1, H Notbohm 1, S Kügler 1, W G Purschke 1, P K Müller 1
PMCID: PMC1217924  PMID: 8947494

Abstract

Vigilin, a protein found predominantly in cells and tissues with a high biosynthetic capacity, was isolated in its native form from human HEp-2 cells (A.T.C.C. CCL23) by immunoaffinity chromatography. Vigilin forms part of a novel ribonucleoprotein complex that also contains additional, as yet uncharacterized, proteins. Experimental evidence suggests that the nucleic acids entrapped in this complex are protected from RNase and belong to the tRNA family. Using either a pool of total human RNA or radioactively labelled tRNA (tRNA (Asp**)) in rebinding experiments, we could show that tRNA is selectively recaptured by the RNA-depleted vigilin-containing complex.

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Selected References

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  1. Ashley C. T., Jr, Wilkinson K. D., Reines D., Warren S. T. FMR1 protein: conserved RNP family domains and selective RNA binding. Science. 1993 Oct 22;262(5133):563–566. doi: 10.1126/science.7692601. [DOI] [PubMed] [Google Scholar]
  2. Burd C. G., Dreyfuss G. Conserved structures and diversity of functions of RNA-binding proteins. Science. 1994 Jul 29;265(5172):615–621. doi: 10.1126/science.8036511. [DOI] [PubMed] [Google Scholar]
  3. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  4. Delahodde A., Becam A. M., Perea J., Jacq C. A yeast protein HX has homologies with the histone H2AF expressed in chicken embryo. Nucleic Acids Res. 1986 Nov 25;14(22):9213–9214. doi: 10.1093/nar/14.22.9213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gibson T. J., Thompson J. D., Heringa J. The KH domain occurs in a diverse set of RNA-binding proteins that include the antiterminator NusA and is probably involved in binding to nucleic acid. FEBS Lett. 1993 Jun 21;324(3):361–366. doi: 10.1016/0014-5793(93)80152-k. [DOI] [PubMed] [Google Scholar]
  6. Henkel B., Schmidt C., Zorbas H., Pöschl E., Gloe T. R., Purschke W. G., Müller P. K. Chicken vigilin gene organization and expression pattern. The domain structure of the protein is reflected by the exon structure. Eur J Biochem. 1992 Oct 1;209(1):321–328. doi: 10.1111/j.1432-1033.1992.tb17292.x. [DOI] [PubMed] [Google Scholar]
  7. Kamma H., Portman D. S., Dreyfuss G. Cell type-specific expression of hnRNP proteins. Exp Cell Res. 1995 Nov;221(1):187–196. doi: 10.1006/excr.1995.1366. [DOI] [PubMed] [Google Scholar]
  8. Kiledjian M., Wang X., Liebhaber S. A. Identification of two KH domain proteins in the alpha-globin mRNP stability complex. EMBO J. 1995 Sep 1;14(17):4357–4364. doi: 10.1002/j.1460-2075.1995.tb00110.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. McKnight G. L., Reasoner J., Gilbert T., Sundquist K. O., Hokland B., McKernan P. A., Champagne J., Johnson C. J., Bailey M. C., Holly R. Cloning and expression of a cellular high density lipoprotein-binding protein that is up-regulated by cholesterol loading of cells. J Biol Chem. 1992 Jun 15;267(17):12131–12141. [PubMed] [Google Scholar]
  11. Neu-Yilik G., Zorbas H., Gloe T. R., Raabe H. M., Hopp-Christensen T. A., Müller P. K. Vigilin is a cytoplasmic protein. A study on its expression in primary cells and in established cell lines of different species. Eur J Biochem. 1993 Apr 15;213(2):727–736. doi: 10.1111/j.1432-1033.1993.tb17813.x. [DOI] [PubMed] [Google Scholar]
  12. Noller H. F., Hoffarth V., Zimniak L. Unusual resistance of peptidyl transferase to protein extraction procedures. Science. 1992 Jun 5;256(5062):1416–1419. doi: 10.1126/science.1604315. [DOI] [PubMed] [Google Scholar]
  13. Plenz G., Gan Y., Raabe H. M., Müller P. K. Expression of vigilin in chicken cartilage and bone. Cell Tissue Res. 1993 Aug;273(2):381–389. doi: 10.1007/BF00312841. [DOI] [PubMed] [Google Scholar]
  14. Pütz J., Puglisi J. D., Florentz C., Giegé R. Identity elements for specific aminoacylation of yeast tRNA(Asp) by cognate aspartyl-tRNA synthetase. Science. 1991 Jun 21;252(5013):1696–1699. doi: 10.1126/science.2047878. [DOI] [PubMed] [Google Scholar]
  15. Schmidt C., Henkel B., Pöschl E., Zorbas H., Purschke W. G., Gloe T. R., Müller P. K. Complete cDNA sequence of chicken vigilin, a novel protein with amplified and evolutionary conserved domains. Eur J Biochem. 1992 Jun 15;206(3):625–634. doi: 10.1111/j.1432-1033.1992.tb16967.x. [DOI] [PubMed] [Google Scholar]
  16. Siomi H., Choi M., Siomi M. C., Nussbaum R. L., Dreyfuss G. Essential role for KH domains in RNA binding: impaired RNA binding by a mutation in the KH domain of FMR1 that causes fragile X syndrome. Cell. 1994 Apr 8;77(1):33–39. doi: 10.1016/0092-8674(94)90232-1. [DOI] [PubMed] [Google Scholar]

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