Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1997 Apr 1;323(Pt 1):147–149. doi: 10.1042/bj3230147

Glutathione S-transferase Yc cDNA from Syrian hamster kidney.

F Maggouta 1, S A Li 1, J J Li 1, J S Norris 1
PMCID: PMC1218287  PMID: 9173874

Abstract

A cDNA encoding alpha-class glutathione S-transferase Yc (GSTYc) has been isolated from a Syrian hamster kidney library, and its nucleotide sequence (968 bp) has been determined. Analysis of the deduced amino acid sequence revealed a high level of identity between Syrian hamster GSTYc, rat GST Yc1 and Yc2 and mouse GSTYc. Northern-blot experiments demonstrated that Syrian hamster GSTYc expression is tissue-specific. A GSTYc mRNA of approx. 1 kb is expressed in liver, kidney, vas deferens and epididymis. Expression of the GSTYc transcript was not detected in testis or uterus.

Full Text

The Full Text of this article is available as a PDF (380.9 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bogaards J. J., van Ommen B., van Bladeren P. J. Purification and characterization of eight glutathione S-transferase isoenzymes of hamster. Comparison of subunit composition of enzymes from liver, kidney, testis, pancreas and trachea. Biochem J. 1992 Sep 1;286(Pt 2):383–388. doi: 10.1042/bj2860383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Buetler T. M., Eaton D. L. Complementary DNA cloning, messenger RNA expression, and induction of alpha-class glutathione S-transferases in mouse tissues. Cancer Res. 1992 Jan 15;52(2):314–318. [PubMed] [Google Scholar]
  3. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  4. Coles B., Ketterer B. The role of glutathione and glutathione transferases in chemical carcinogenesis. Crit Rev Biochem Mol Biol. 1990;25(1):47–70. doi: 10.3109/10409239009090605. [DOI] [PubMed] [Google Scholar]
  5. Daniel V., Sharon R., Tichauer Y., Sarid S. Mouse glutathione S-transferase Ya subunit: gene structure and sequence. DNA. 1987 Aug;6(4):317–324. doi: 10.1089/dna.1987.6.317. [DOI] [PubMed] [Google Scholar]
  6. Hayes J. D., Judah D. J., McLellan L. I., Kerr L. A., Peacock S. D., Neal G. E. Ethoxyquin-induced resistance to aflatoxin B1 in the rat is associated with the expression of a novel alpha-class glutathione S-transferase subunit, Yc2, which possesses high catalytic activity for aflatoxin B1-8,9-epoxide. Biochem J. 1991 Oct 15;279(Pt 2):385–398. doi: 10.1042/bj2790385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hayes J. D., Nguyen T., Judah D. J., Petersson D. G., Neal G. E. Cloning of cDNAs from fetal rat liver encoding glutathione S-transferase Yc polypeptides. The Yc2 subunit is expressed in adult rat liver resistant to the hepatocarcinogen aflatoxin B1. J Biol Chem. 1994 Aug 12;269(32):20707–20717. [PubMed] [Google Scholar]
  8. Hayes J. D., Pulford D. J. The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Crit Rev Biochem Mol Biol. 1995;30(6):445–600. doi: 10.3109/10409239509083491. [DOI] [PubMed] [Google Scholar]
  9. Jensen D. E., Mackay R. L. Rat, mouse and hamster isozyme specificity in the glutathione transferase-mediated denitrosation of nitrosoguanidinium compounds. Cancer Res. 1990 Mar 1;50(5):1440–1448. [PubMed] [Google Scholar]
  10. Lai H. C., Li N., Weiss M. J., Reddy C. C., Tu C. P. The nucleotide sequence of a rat liver glutathione S-transferase subunit cDNA clone. J Biol Chem. 1984 May 10;259(9):5536–5542. [PubMed] [Google Scholar]
  11. Li N. Q., Tu C. P. Suppression of glutathione S-transferases in rat seminal vesicles or pituitary glands. Biochem Biophys Res Commun. 1986 May 14;136(3):1057–1062. doi: 10.1016/0006-291x(86)90440-7. [DOI] [PubMed] [Google Scholar]
  12. Mannervik B. The isoenzymes of glutathione transferase. Adv Enzymol Relat Areas Mol Biol. 1985;57:357–417. doi: 10.1002/9780470123034.ch5. [DOI] [PubMed] [Google Scholar]
  13. Meyer D. J., Coles B., Pemble S. E., Gilmore K. S., Fraser G. M., Ketterer B. Theta, a new class of glutathione transferases purified from rat and man. Biochem J. 1991 Mar 1;274(Pt 2):409–414. doi: 10.1042/bj2740409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Meyer D. J., Thomas M. Characterization of rat spleen prostaglandin H D-isomerase as a sigma-class GSH transferase. Biochem J. 1995 Nov 1;311(Pt 3):739–742. doi: 10.1042/bj3110739. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pearson W. R., Reinhart J., Sisk S. C., Anderson K. S., Adler P. N. Tissue-specific induction of murine glutathione transferase mRNAs by butylated hydroxyanisole. J Biol Chem. 1988 Sep 15;263(26):13324–13332. [PubMed] [Google Scholar]
  16. Pickett C. B., Telakowski-Hopkins C. A., Ding G. J., Argenbright L., Lu A. Y. Rat liver glutathione S-transferases. Complete nucleotide sequence of a glutathione S-transferase mRNA and the regulation of the Ya, Yb, and Yc mRNAs by 3-methylcholanthrene and phenobarbital. J Biol Chem. 1984 Apr 25;259(8):5182–5188. [PubMed] [Google Scholar]
  17. Pulford D. J., Hayes J. D. Characterization of the rat glutathione S-transferase Yc2 subunit gene, GSTA5: identification of a putative antioxidant-responsive element in the 5'-flanking region of rat GSTA5 that may mediate chemoprotection against aflatoxin B1. Biochem J. 1996 Aug 15;318(Pt 1):75–84. doi: 10.1042/bj3180075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stenberg G., Ridderström M., Engström A., Pemble S. E., Mannervik B. Cloning and heterologous expression of cDNA encoding class alpha rat glutathione transferase 8-8, an enzyme with high catalytic activity towards genotoxic alpha,beta-unsaturated carbonyl compounds. Biochem J. 1992 Jun 1;284(Pt 2):313–319. doi: 10.1042/bj2840313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Telakowski-Hopkins C. A., Rodkey J. A., Bennett C. D., Lu A. Y., Pickett C. B. Rat liver glutathione S-transferases. Construction of a cDNA clone complementary to a Yc mRNA and prediction of the complete amino acid sequence of a Yc subunit. J Biol Chem. 1985 May 10;260(9):5820–5825. [PubMed] [Google Scholar]
  20. Telakowski-Hopkins C. A., Rothkopf G. S., Pickett C. B. Structural analysis of a rat liver glutathione S-transferase Ya gene. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9393–9397. doi: 10.1073/pnas.83.24.9393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tu C. P., Lai H. C., Li N. Q., Weiss M. J., Reddy C. C. The Yc and Ya subunits of rat liver glutathione S-transferases are the products of separate genes. J Biol Chem. 1984 Aug 10;259(15):9434–9439. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES