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. 1997 May 15;324(Pt 1):347–352. doi: 10.1042/bj3240347

Chemoattractants induce tyrosine phosphorylation of ERK2 in Dictyostelium discoideum by diverse signalling pathways.

C Kosaka 1, C J Pears 1
PMCID: PMC1218436  PMID: 9164876

Abstract

Two homologues of mitogen-activated protein kinases have been identified in Dictyostelium discoideum (ERK1 and EKR2). We here demonstrate transient tyrosine phosphorylation of ERK2 in response to the chemoattractants cAMP and folic acid that correlates with activity. To investigate the signalling pathways, we studied the response in strains with altered cAMP-dependent protein kinase (PKA) status. The degree of cAMP-induced ERK2 tyrosine phosphorylation was increased in cells overexpressing PKA activity but no such increase was observed in the response to folic acid. Our observations suggest that cAMP-induced ERK2 tyrosine phosphorylation is positively modulated by a PKA-regulated step which is not involved in the response to folic acid, suggesting the presence of diverse signalling pathways leading to ERK2 activation.

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Selected References

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  1. Adams P. D., Parker P. J. TPA-induced activation of MAP kinase. FEBS Lett. 1991 Sep 23;290(1-2):77–82. doi: 10.1016/0014-5793(91)81230-6. [DOI] [PubMed] [Google Scholar]
  2. Anjard C., Pinaud S., Kay R. R., Reymond C. D. Overexpression of Dd PK2 protein kinase causes rapid development and affects the intracellular cAMP pathway of Dictyostelium discoideum. Development. 1992 Jul;115(3):785–790. doi: 10.1242/dev.115.3.785. [DOI] [PubMed] [Google Scholar]
  3. Chen M. Y., Insall R. H., Devreotes P. N. Signaling through chemoattractant receptors in Dictyostelium. Trends Genet. 1996 Feb;12(2):52–57. doi: 10.1016/0168-9525(96)81400-4. [DOI] [PubMed] [Google Scholar]
  4. Frödin M., Peraldi P., Van Obberghen E. Cyclic AMP activates the mitogen-activated protein kinase cascade in PC12 cells. J Biol Chem. 1994 Feb 25;269(8):6207–6214. [PubMed] [Google Scholar]
  5. Gaskins C., Maeda M., Firtel R. A. Identification and functional analysis of a developmentally regulated extracellular signal-regulated kinase gene in Dictyostelium discoideum. Mol Cell Biol. 1994 Oct;14(10):6996–7012. doi: 10.1128/mcb.14.10.6996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hadwiger J. A., Lee S., Firtel R. A. The G alpha subunit G alpha 4 couples to pterin receptors and identifies a signaling pathway that is essential for multicellular development in Dictyostelium. Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10566–10570. doi: 10.1073/pnas.91.22.10566. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Harwood A. J., Hopper N. A., Simon M. N., Bouzid S., Veron M., Williams J. G. Multiple roles for cAMP-dependent protein kinase during Dictyostelium development. Dev Biol. 1992 Jan;149(1):90–99. doi: 10.1016/0012-1606(92)90266-j. [DOI] [PubMed] [Google Scholar]
  8. Hordijk P. L., Verlaan I., Jalink K., van Corven E. J., Moolenaar W. H. cAMP abrogates the p21ras-mitogen-activated protein kinase pathway in fibroblasts. J Biol Chem. 1994 Feb 4;269(5):3534–3538. [PubMed] [Google Scholar]
  9. Kameshita I., Fujisawa H. A sensitive method for detection of calmodulin-dependent protein kinase II activity in sodium dodecyl sulfate-polyacrylamide gel. Anal Biochem. 1989 Nov 15;183(1):139–143. doi: 10.1016/0003-2697(89)90181-4. [DOI] [PubMed] [Google Scholar]
  10. Knetsch M. L., Epskamp S. J., Schenk P. W., Wang Y., Segall J. E., Snaar-Jagalska B. E. Dual role of cAMP and involvement of both G-proteins and ras in regulation of ERK2 in Dictyostelium discoideum. EMBO J. 1996 Jul 1;15(13):3361–3368. [PMC free article] [PubMed] [Google Scholar]
  11. Kumagai A., Hadwiger J. A., Pupillo M., Firtel R. A. Molecular genetic analysis of two G alpha protein subunits in Dictyostelium. J Biol Chem. 1991 Jan 15;266(2):1220–1228. [PubMed] [Google Scholar]
  12. Maeda M., Aubry L., Insall R., Gaskins C., Devreotes P. N., Firtel R. A. Seven helix chemoattractant receptors transiently stimulate mitogen-activated protein kinase in Dictyostelium. Role of heterotrimeric G proteins. J Biol Chem. 1996 Feb 16;271(7):3351–3354. doi: 10.1074/jbc.271.7.3351. [DOI] [PubMed] [Google Scholar]
  13. Mann S. K., Yonemoto W. M., Taylor S. S., Firtel R. A. DdPK3, which plays essential roles during Dictyostelium development, encodes the catalytic subunit of cAMP-dependent protein kinase. Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10701–10705. doi: 10.1073/pnas.89.22.10701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Marshall C. J. MAP kinase kinase kinase, MAP kinase kinase and MAP kinase. Curr Opin Genet Dev. 1994 Feb;4(1):82–89. doi: 10.1016/0959-437x(94)90095-7. [DOI] [PubMed] [Google Scholar]
  15. Pitt G. S., Brandt R., Lin K. C., Devreotes P. N., Schaap P. Extracellular cAMP is sufficient to restore developmental gene expression and morphogenesis in Dictyostelium cells lacking the aggregation adenylyl cyclase (ACA). Genes Dev. 1993 Nov;7(11):2172–2180. doi: 10.1101/gad.7.11.2172. [DOI] [PubMed] [Google Scholar]
  16. Schulkes C., Schaap P. cAMP-dependent protein kinase activity is essential for preaggregative gene expression in Dictyostelium. FEBS Lett. 1995 Jul 17;368(2):381–384. doi: 10.1016/0014-5793(95)00676-z. [DOI] [PubMed] [Google Scholar]
  17. Segall J. E., Kuspa A., Shaulsky G., Ecke M., Maeda M., Gaskins C., Firtel R. A., Loomis W. F. A MAP kinase necessary for receptor-mediated activation of adenylyl cyclase in Dictyostelium. J Cell Biol. 1995 Feb;128(3):405–413. doi: 10.1083/jcb.128.3.405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Simon M. N., Pelegrini O., Veron M., Kay R. R. Mutation of protein kinase A causes heterochronic development of Dictyostelium. Nature. 1992 Mar 12;356(6365):171–172. doi: 10.1038/356171a0. [DOI] [PubMed] [Google Scholar]
  19. Watts D. J., Ashworth J. M. Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture. Biochem J. 1970 Sep;119(2):171–174. doi: 10.1042/bj1190171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wu L., Valkema R., Van Haastert P. J., Devreotes P. N. The G protein beta subunit is essential for multiple responses to chemoattractants in Dictyostelium. J Cell Biol. 1995 Jun;129(6):1667–1675. doi: 10.1083/jcb.129.6.1667. [DOI] [PMC free article] [PubMed] [Google Scholar]

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