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. 1997 Aug 15;326(Pt 1):93–98. doi: 10.1042/bj3260093

Involvement of proteasomal subunits zeta and iota in RNA degradation.

F Petit 1, A S Jarrousse 1, B Dahlmann 1, A Sobek 1, K B Hendil 1, J Buri 1, Y Briand 1, H P Schmid 1
PMCID: PMC1218641  PMID: 9337855

Abstract

We have identified two distinct subunits of 20 S proteasomes that are associated with RNase activity. Proteasome subunits zeta and iota, eluted from two-dimensional Western blots, hydrolysed tobacco mosaic virus RNA, whereas none of the other subunits degraded this substrate under the same conditions. Additionally, proteasomes were dissociated by 6 M urea, and subunit zeta, containing the highest RNase activity, was isolated by anion-exchange chromatography and gel filtration. Purified subunit zeta migrated as a single spot on two-dimensional PAGE with a molecular mass of approx. 28 kDa. Addition of anti-(subunit zeta) antibodies led to the co-precipitation of this proteasome subunit and nuclease activity. This is the first evidence that proteasomal alpha-type subunits are associated with an enzymic activity, and our results provide further evidence that proteasomes may be involved in cellular RNA metabolism.

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Selected References

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