Figure 1.

Representative current traces and I-V relationships of the CAN channels and cloned TRPM4 and TRPM5 channels. A&B, Example single-channel recordings of calcium-activated non-selective cation channels from cultured cardiac cells (A) and their I-V relationship (B). Recording voltage was −70 mV; intracellular calcium concentration was 6 μM (top), 1.5 μM (middle), and zero μM (bottom). Filled circles and open squares in B are data points with symmetrical sodium saline solutions and with intracellular potassium/extracellular sodium saline solutions, respectively, with reversal potentials around zero mV. Reproduced from Colquhoun et al. (5); used with permission under CC-BY license. C&D, TRPM4 single-channel currents activated by 0.3 μM calcium (C) and their I-V relationship with intracellular potassium/extracellular sodium saline solutions (D), with a reversal potential around zero mV. Reproduced from Launay et al. (13) with permission. E&F, TRPM5 currents activated by 12 μM calcium (E) and the I-V relationship (F). Reproduced from Liu and Liman (20) with permission.