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. 1997 Oct 1;327(Pt 1):17–21. doi: 10.1042/bj3270017

A novel procedure for the efficient purification of the cystic fibrosis transmembrane conductance regulator (CFTR).

M Ramjeesingh 1, C Li 1, E Garami 1, L J Huan 1, M Hewryk 1, Y Wang 1, K Galley 1, C E Bear 1
PMCID: PMC1218756  PMID: 9355728

Abstract

This report describes a novel, single-step strategy for the purification of the cystic fibrosis transmembrane conductance regulator from Sf9 cells, which will facilitate studies of the structure-function relationships of this clinically important molecule. The new method combines the use of the novel detergent sodium pentadecafluoro-octanoate with metal-affinity chromatography to produce a high yield of purified protein which can be functionally reconstituted as a chloride channel and an ATPase.

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Selected References

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