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. 1997 Nov 15;328(Pt 1):69–74. doi: 10.1042/bj3280069

Regulation of human prohormone convertase 2 promoter activity by the transcription factor EGR-1.

E Jansen 1, T A Ayoubi 1, S M Meulemans 1, W J Van De Ven 1
PMCID: PMC1218888  PMID: 9359835

Abstract

Prohormone convertases are involved in the tissue-specific endoproteolytic processing of prohormones and neuropeptide precursors within the secretory pathway. In the present study, we have isolated genomic clones comprising the 5'-terminal region of the human prohormone convertase 2 (PC2) gene and established characteristics of the PC2 promoter region. The proximal promoter region is very G+C-rich and does not contain a canonical TATA box or a CAAT box. Transient expression assays with a set of human PC2 gene fragments containing progressive 5' deletions demonstrate that the proximal promoter region is capable of directing high levels of neuroendocrine-specific expression of reporter gene constructs. In addition, we show that the transcription factor EGR-1 interacts with two distinct elements within the proximal human PC2 promoter region. Transfection experiments also demonstrate that EGR-1 is able to enhance PC2 promoter activity.

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Selected References

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