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. 1998 Jul 1;333(Pt 1):1–3. doi: 10.1042/bj3330001

Prothymosin alpha is a chromatin-remodelling protein in mammalian cells.

J Gomez-Marquez 1, P Rodríguez 1
PMCID: PMC1219547  PMID: 9639554

Abstract

Prothymosin alpha (ProTalpha) is an abundant mammalian acidic nuclear protein whose expression is related to cell proliferation. Here we report that in HL-60 cells overexpressing ProTalpha, the accessibility of micrococcal nuclease to chromatin is strongly increased. In the DNA ladder generated by the nuclease activity, the sizes of the mononucleosome (146 bp, the DNA fragment that is bound to the histone octamer) and its multimers correspond to nucleosomes lacking histone H1. The percentage of histone-H1-depleted chromatin (active chromatin) is also higher in the cells overexpressing ProTalpha. On the basis of these and previous findings, we propose a biological role for ProTalpha in the remodelling of chromatin fibres through its interaction with histone H1.

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Selected References

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  1. Bustelo X. R., Otero A., Gómez-Márquez J., Freire M. Expression of the rat prothymosin alpha gene during T-lymphocyte proliferation and liver regeneration. J Biol Chem. 1991 Jan 25;266(3):1443–1447. [PubMed] [Google Scholar]
  2. Cairns B. R. Chromatin remodeling machines: similar motors, ulterior motives. Trends Biochem Sci. 1998 Jan;23(1):20–25. doi: 10.1016/s0968-0004(97)01160-2. [DOI] [PubMed] [Google Scholar]
  3. Díaz-Jullien C., Pérez-Estévez A., Covelo G., Freire M. Prothymosin alpha binds histones in vitro and shows activity in nucleosome assembly assay. Biochim Biophys Acta. 1996 Sep 5;1296(2):219–227. doi: 10.1016/0167-4838(96)00072-6. [DOI] [PubMed] [Google Scholar]
  4. Earnshaw W. C. Anionic regions in nuclear proteins. J Cell Biol. 1987 Oct;105(4):1479–1482. doi: 10.1083/jcb.105.4.1479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eilers M., Schirm S., Bishop J. M. The MYC protein activates transcription of the alpha-prothymosin gene. EMBO J. 1991 Jan;10(1):133–141. doi: 10.1002/j.1460-2075.1991.tb07929.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Eschenfeldt W. H., Berger S. L. The human prothymosin alpha gene is polymorphic and induced upon growth stimulation: evidence using a cloned cDNA. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9403–9407. doi: 10.1073/pnas.83.24.9403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Felsenfeld G. Chromatin as an essential part of the transcriptional mechanism. Nature. 1992 Jan 16;355(6357):219–224. doi: 10.1038/355219a0. [DOI] [PubMed] [Google Scholar]
  8. Gómez-Márquez J., Segade F., Dosil M., Pichel J. G., Bustelo X. R., Freire M. The expression of prothymosin alpha gene in T lymphocytes and leukemic lymphoid cells is tied to lymphocyte proliferation. J Biol Chem. 1989 May 25;264(15):8451–8454. [PubMed] [Google Scholar]
  9. Gómez-Márquez J., Segade F. Prothymosin alpha is a nuclear protein. FEBS Lett. 1988 Jan 4;226(2):217–219. doi: 10.1016/0014-5793(88)81425-x. [DOI] [PubMed] [Google Scholar]
  10. Keene M. A., Elgin S. C. Micrococcal nuclease as a probe of DNA sequence organization and chromatin structure. Cell. 1981 Nov;27(1 Pt 2):57–64. doi: 10.1016/0092-8674(81)90360-3. [DOI] [PubMed] [Google Scholar]
  11. Levy-Wilson B., Fortier C., Blackhart B. D., McCarthy B. J. DNase I- and micrococcal nuclease-hypersensitive sites in the human apolipoprotein B gene are tissue specific. Mol Cell Biol. 1988 Jan;8(1):71–80. doi: 10.1128/mcb.8.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Luger K., Mäder A. W., Richmond R. K., Sargent D. F., Richmond T. J. Crystal structure of the nucleosome core particle at 2.8 A resolution. Nature. 1997 Sep 18;389(6648):251–260. doi: 10.1038/38444. [DOI] [PubMed] [Google Scholar]
  13. Lutz W., Stöhr M., Schürmann J., Wenzel A., Löhr A., Schwab M. Conditional expression of N-myc in human neuroblastoma cells increases expression of alpha-prothymosin and ornithine decarboxylase and accelerates progression into S-phase early after mitogenic stimulation of quiescent cells. Oncogene. 1996 Aug 15;13(4):803–812. [PubMed] [Google Scholar]
  14. Manrow R. E., Sburlati A. R., Hanover J. A., Berger S. L. Nuclear targeting of prothymosin alpha. J Biol Chem. 1991 Feb 25;266(6):3916–3924. [PubMed] [Google Scholar]
  15. Mills A. D., Laskey R. A., Black P., De Robertis E. M. An acidic protein which assembles nucleosomes in vitro is the most abundant protein in Xenopus oocyte nuclei. J Mol Biol. 1980 May 25;139(3):561–568. doi: 10.1016/0022-2836(80)90148-5. [DOI] [PubMed] [Google Scholar]
  16. Papamarcaki T., Tsolas O. Prothymosin alpha binds to histone H1 in vitro. FEBS Lett. 1994 May 23;345(1):71–75. doi: 10.1016/0014-5793(94)00439-0. [DOI] [PubMed] [Google Scholar]
  17. Rodríguez P., Viñuela J. E., Alvarez-Fernández L., Buceta M., Vidal A., Domínguez F., Gómez-Márquez J. Overexpression of prothymosin alpha accelerates proliferation and retards differentiation in HL-60 cells. Biochem J. 1998 May 1;331(Pt 3):753–761. doi: 10.1042/bj3310753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sburlati A. R., Manrow R. E., Berger S. L. Human prothymosin alpha: purification of a highly acidic nuclear protein by means of a phenol extraction. Protein Expr Purif. 1990 Nov;1(2):184–190. doi: 10.1016/1046-5928(90)90014-p. [DOI] [PubMed] [Google Scholar]
  19. Sburlati A. R., Manrow R. E., Berger S. L. Prothymosin alpha antisense oligomers inhibit myeloma cell division. Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):253–257. doi: 10.1073/pnas.88.1.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Trumbore M. W., Wang R. H., Enkemann S. A., Berger S. L. Prothymosin alpha in vivo contains phosphorylated glutamic acid residues. J Biol Chem. 1997 Oct 17;272(42):26394–26404. doi: 10.1074/jbc.272.42.26394. [DOI] [PubMed] [Google Scholar]
  21. Wang R. H., Tao L., Trumbore M. W., Berger S. L. Turnover of the acyl phosphates of human and murine prothymosin alpha in vivo. J Biol Chem. 1997 Oct 17;272(42):26405–26412. doi: 10.1074/jbc.272.42.26405. [DOI] [PubMed] [Google Scholar]
  22. Watts J. D., Cary P. D., Crane-Robinson C. Prothymosin alpha is a nuclear protein. FEBS Lett. 1989 Mar 13;245(1-2):17–20. doi: 10.1016/0014-5793(89)80182-6. [DOI] [PubMed] [Google Scholar]
  23. Zhao K., Käs E., Gonzalez E., Laemmli U. K. SAR-dependent mobilization of histone H1 by HMG-I/Y in vitro: HMG-I/Y is enriched in H1-depleted chromatin. EMBO J. 1993 Aug;12(8):3237–3247. doi: 10.1002/j.1460-2075.1993.tb05993.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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