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. 1998 Aug 1;333(Pt 3):735–739. doi: 10.1042/bj3330735

Amino acid sequence of glutathione S-transferase rGSTM5* from rat testis.

M F Tam 1, C H Hsieh 1, S P Tsai 1, T C Tam 1
PMCID: PMC1219639  PMID: 9677335

Abstract

Glutathione S-transferase rGSTM5* was isolated from rat testis with a combination of glutathione affinity column and reverse-phase column chromatography. The protein was digested with Achromobacter protease I or endoproteinase Arg-C. The peptide fragments were isolated for electrospray MS and N-terminal peptide sequencing analyses. The primary amino acid sequence of rGSTM5* comprises 217 residues and has a calculated average molecular mass of 25495.3 Da. The result is identical to that obtained for rGSTM5* with liquid chromatography-MS from a mixture of rat testicular GSTs. Therefore, rGSTM5* has not been post-translationally modified.

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Selected References

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