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. 1998 Oct 15;335(Pt 2):301–304. doi: 10.1042/bj3350301

Evidence for the presence of multiple forms of Sph kinase in human platelets.

Y Banno 1, M Kato 1, A Hara 1, Y Nozawa 1
PMCID: PMC1219782  PMID: 9761727

Abstract

The intracellular distribution of sphingosine (Sph) kinase activity was examined in human platelets. A large proportion (72%) of the total activity was found to be associated with the membrane fraction, and the membrane-associated fraction had higher specific activity compared with the cytosolic enzyme. Most of the membrane-associated activity could be extracted with 1 M NaCl. The cytosolic activity was unstable upon heat treatment, with 80% of the activity being lost during incubation at 45 degreesC for 1 h, whereas the NaCl-extractable fraction was stable under the same conditions. When subjected to Mono Q column chromatography, the cytosolic fraction produced two activity peaks and the NaCl-extractable fraction gave a single peak. These three Sph kinase activities showed different responses to stimulation by beta-octylglucoside and inhibition by N, N-dimethylsphingosine and l-threo-dihydrosphingosine, suggesting the presence of multiple enzyme forms in human platelets.

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Selected References

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  1. Buehrer B. M., Bell R. M. Inhibition of sphingosine kinase in vitro and in platelets. Implications for signal transduction pathways. J Biol Chem. 1992 Feb 15;267(5):3154–3159. [PubMed] [Google Scholar]
  2. Buehrer B. M., Bell R. M. Sphingosine kinase: properties and cellular functions. Adv Lipid Res. 1993;26:59–67. [PubMed] [Google Scholar]
  3. Choi O. H., Kim J. H., Kinet J. P. Calcium mobilization via sphingosine kinase in signalling by the Fc epsilon RI antigen receptor. Nature. 1996 Apr 18;380(6575):634–636. doi: 10.1038/380634a0. [DOI] [PubMed] [Google Scholar]
  4. Desai N. N., Zhang H., Olivera A., Mattie M. E., Spiegel S. Sphingosine-1-phosphate, a metabolite of sphingosine, increases phosphatidic acid levels by phospholipase D activation. J Biol Chem. 1992 Nov 15;267(32):23122–23128. [PubMed] [Google Scholar]
  5. Ghosh T. K., Bian J., Gill D. L. Intracellular calcium release mediated by sphingosine derivatives generated in cells. Science. 1990 Jun 29;248(4963):1653–1656. doi: 10.1126/science.2163543. [DOI] [PubMed] [Google Scholar]
  6. Gómez-Muñoz A., Waggoner D. W., O'Brien L., Brindley D. N. Interaction of ceramides, sphingosine, and sphingosine 1-phosphate in regulating DNA synthesis and phospholipase D activity. J Biol Chem. 1995 Nov 3;270(44):26318–26325. doi: 10.1074/jbc.270.44.26318. [DOI] [PubMed] [Google Scholar]
  7. Hannun Y. A., Bell R. M. Functions of sphingolipids and sphingolipid breakdown products in cellular regulation. Science. 1989 Jan 27;243(4890):500–507. doi: 10.1126/science.2643164. [DOI] [PubMed] [Google Scholar]
  8. Kolesnick R., Golde D. W. The sphingomyelin pathway in tumor necrosis factor and interleukin-1 signaling. Cell. 1994 May 6;77(3):325–328. doi: 10.1016/0092-8674(94)90147-3. [DOI] [PubMed] [Google Scholar]
  9. Lee M. J., Van Brocklyn J. R., Thangada S., Liu C. H., Hand A. R., Menzeleev R., Spiegel S., Hla T. Sphingosine-1-phosphate as a ligand for the G protein-coupled receptor EDG-1. Science. 1998 Mar 6;279(5356):1552–1555. doi: 10.1126/science.279.5356.1552. [DOI] [PubMed] [Google Scholar]
  10. Louie D. D., Kisic A., Schroefer G. J., Jr Sphingolipid base metabolism. Partial purification and properties of sphinganine kinase of brain. J Biol Chem. 1976 Aug 10;251(15):4557–4564. [PubMed] [Google Scholar]
  11. Merrill A. H., Jr, Stevens V. L. Modulation of protein kinase C and diverse cell functions by sphingosine--a pharmacologically interesting compound linking sphingolipids and signal transduction. Biochim Biophys Acta. 1989 Feb 9;1010(2):131–139. doi: 10.1016/0167-4889(89)90152-3. [DOI] [PubMed] [Google Scholar]
  12. Meyer zu Heringdorf D., van Koppen C. J., Jakobs K. H. Molecular diversity of sphingolipid signalling. FEBS Lett. 1997 Jun 23;410(1):34–38. doi: 10.1016/s0014-5793(97)00320-7. [DOI] [PubMed] [Google Scholar]
  13. Olivera A., Rosenthal J., Spiegel S. Effect of acidic phospholipids on sphingosine kinase. J Cell Biochem. 1996 Mar 15;60(4):529–537. doi: 10.1002/(sici)1097-4644(19960315)60:4<529::aid-jcb9>3.0.co;2-u. [DOI] [PubMed] [Google Scholar]
  14. Olivera A., Rosenthal J., Spiegel S. Sphingosine kinase from Swiss 3T3 fibroblasts: a convenient assay for the measurement of intracellular levels of free sphingoid bases. Anal Biochem. 1994 Dec;223(2):306–312. doi: 10.1006/abio.1994.1589. [DOI] [PubMed] [Google Scholar]
  15. Olivera A., Spiegel S. Sphingosine-1-phosphate as second messenger in cell proliferation induced by PDGF and FCS mitogens. Nature. 1993 Oct 7;365(6446):557–560. doi: 10.1038/365557a0. [DOI] [PubMed] [Google Scholar]
  16. Rani C. S., Wang F., Fuior E., Berger A., Wu J., Sturgill T. W., Beitner-Johnson D., LeRoith D., Varticovski L., Spiegel S. Divergence in signal transduction pathways of platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) receptors. Involvement of sphingosine 1-phosphate in PDGF but not EGF signaling. J Biol Chem. 1997 Apr 18;272(16):10777–10783. doi: 10.1074/jbc.272.16.10777. [DOI] [PubMed] [Google Scholar]
  17. Spiegel S., Olivera A., Carlson R. O. The role of sphingosine in cell growth regulation and transmembrane signaling. Adv Lipid Res. 1993;25:105–129. [PubMed] [Google Scholar]
  18. Stoffel W., Bauer E., Stahl J. The metabolism of sphingosine bases in Tetrahymena pyriformis. Sphingosine kinase and sphingosine-1-phosphate lyase. Hoppe Seylers Z Physiol Chem. 1974 Jan;355(1):61–74. doi: 10.1515/bchm2.1974.355.1.61. [DOI] [PubMed] [Google Scholar]
  19. Stoffel W., Heimann G., Hellenbroich B. Sphingosine kinase in blood platelets. Hoppe Seylers Z Physiol Chem. 1973 May;354(5):562–566. doi: 10.1515/bchm2.1973.354.1.562. [DOI] [PubMed] [Google Scholar]
  20. Stoffel W., Hellenbroich B., Heimann G. Properties and specificities of sphingosine kinase from blood platelets. Hoppe Seylers Z Physiol Chem. 1973 Oct-Nov;354(10-11):1311–1316. doi: 10.1515/bchm2.1973.354.2.1311. [DOI] [PubMed] [Google Scholar]
  21. Yatomi Y., Igarashi Y., Yang L., Hisano N., Qi R., Asazuma N., Satoh K., Ozaki Y., Kume S. Sphingosine 1-phosphate, a bioactive sphingolipid abundantly stored in platelets, is a normal constituent of human plasma and serum. J Biochem. 1997 May;121(5):969–973. doi: 10.1093/oxfordjournals.jbchem.a021681. [DOI] [PubMed] [Google Scholar]
  22. Yatomi Y., Ruan F., Hakomori S., Igarashi Y. Sphingosine-1-phosphate: a platelet-activating sphingolipid released from agonist-stimulated human platelets. Blood. 1995 Jul 1;86(1):193–202. [PubMed] [Google Scholar]
  23. Yatomi Y., Ruan F., Megidish T., Toyokuni T., Hakomori S., Igarashi Y. N,N-dimethylsphingosine inhibition of sphingosine kinase and sphingosine 1-phosphate activity in human platelets. Biochemistry. 1996 Jan 16;35(2):626–633. doi: 10.1021/bi9515533. [DOI] [PubMed] [Google Scholar]
  24. Yatomi Y., Welch R. J., Igarashi Y. Distribution of sphingosine 1-phosphate, a bioactive sphingolipid, in rat tissues. FEBS Lett. 1997 Mar 10;404(2-3):173–174. doi: 10.1016/s0014-5793(97)00121-x. [DOI] [PubMed] [Google Scholar]
  25. Yatomi Y., Yamamura S., Ruan F., Igarashi Y. Sphingosine 1-phosphate induces platelet activation through an extracellular action and shares a platelet surface receptor with lysophosphatidic acid. J Biol Chem. 1997 Feb 21;272(8):5291–5297. doi: 10.1074/jbc.272.8.5291. [DOI] [PubMed] [Google Scholar]
  26. Zondag G. C., Postma F. R., Etten I. V., Verlaan I., Moolenaar W. H. Sphingosine 1-phosphate signalling through the G-protein-coupled receptor Edg-1. Biochem J. 1998 Mar 1;330(Pt 2):605–609. doi: 10.1042/bj3300605. [DOI] [PMC free article] [PubMed] [Google Scholar]

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