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. 1998 Oct 15;335(Pt 2):405–408. doi: 10.1042/bj3350405

Glucose induces expression of stearoyl-CoA desaturase in 3T3-L1 adipocytes.

B H Jones 1, M K Standridge 1, K J Claycombe 1, P J Smith 1, N Moustaïd-Moussa 1
PMCID: PMC1219803  PMID: 9867800

Abstract

Stearoyl-CoA desaturase (SCD; EC 1.14.99.5) is a key enzyme in the synthesis polyunsaturated fatty acids. Liver and ose tissue are the predominant sites of SCD expression. Regulation of tic SCD by various nutritional and hormonal ors, such as insulin, dietary carbohydrates and polyunsaturated fatty s, has been well documented. Little is known, ver, about adipocyte SCD regulation despite high levels of SCD activity adipose tissue. The present study was gned to investigate SCD regulation in adipocytes by examining the cts of glucose and insulin on SCD expression. We rt here that glucose availability directly increased SCD gene scription in 3T3-L1 adipocytes. This response was pendent of insulin, and insulin alone in the absence of glucose had no ct on SCD mRNA levels. SCD thus represents a l model in which to investigate the mechanisms of direct regulation of expression by glucose in adipose cells.

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Selected References

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  1. Enoch H. G., Catalá A., Strittmatter P. Mechanism of rat liver microsomal stearyl-CoA desaturase. Studies of the substrate specificity, enzyme-substrate interactions, and the function of lipid. J Biol Chem. 1976 Aug 25;251(16):5095–5103. [PubMed] [Google Scholar]
  2. Field C. J., Ryan E. A., Thomson A. B., Clandinin M. T. Diet fat composition alters membrane phospholipid composition, insulin binding, and glucose metabolism in adipocytes from control and diabetic animals. J Biol Chem. 1990 Jul 5;265(19):11143–11150. [PubMed] [Google Scholar]
  3. Foufelle F., Gouhot B., Pégorier J. P., Perdereau D., Girard J., Ferré P. Glucose stimulation of lipogenic enzyme gene expression in cultured white adipose tissue. A role for glucose 6-phosphate. J Biol Chem. 1992 Oct 15;267(29):20543–20546. [PubMed] [Google Scholar]
  4. Hillgartner F. B., Salati L. M., Goodridge A. G. Physiological and molecular mechanisms involved in nutritional regulation of fatty acid synthesis. Physiol Rev. 1995 Jan;75(1):47–76. doi: 10.1152/physrev.1995.75.1.47. [DOI] [PubMed] [Google Scholar]
  5. Jones B. H., Maher M. A., Banz W. J., Zemel M. B., Whelan J., Smith P. J., Moustaïd N. Adipose tissue stearoyl-CoA desaturase mRNA is increased by obesity and decreased by polyunsaturated fatty acids. Am J Physiol. 1996 Jul;271(1 Pt 1):E44–E49. doi: 10.1152/ajpendo.1996.271.1.E44. [DOI] [PubMed] [Google Scholar]
  6. Kitzman H. H., Jr, McMahon R. J., Aslanian A. M., Fadia P. M., Frost S. C. Differential regulation of GRP78 and GLUT1 expression in 3T3-L1 adipocytes. Mol Cell Biochem. 1996 Sep 6;162(1):51–58. doi: 10.1007/BF00250995. [DOI] [PubMed] [Google Scholar]
  7. Kotchen T. A., Reddy S., Zhang H. Y. Increasing insulin sensitivity lowers blood pressure in the fructose-fed rat. Am J Hypertens. 1997 Sep;10(9 Pt 1):1020–1026. doi: 10.1016/s0895-7061(97)00164-7. [DOI] [PubMed] [Google Scholar]
  8. Landschulz K. T., Jump D. B., MacDougald O. A., Lane M. D. Transcriptional control of the stearoyl-CoA desaturase-1 gene by polyunsaturated fatty acids. Biochem Biophys Res Commun. 1994 Apr 29;200(2):763–768. doi: 10.1006/bbrc.1994.1516. [DOI] [PubMed] [Google Scholar]
  9. Legrand P., Bensadoun A. Stearyl-CoA desaturase activity in cultured rat hepatocytes. Biochim Biophys Acta. 1991 Oct 15;1086(1):89–94. doi: 10.1016/0005-2760(91)90158-e. [DOI] [PubMed] [Google Scholar]
  10. Luo J., Rizkalla S. W., Lerer-Metzger M., Boillot J., Ardeleanu A., Bruzzo F., Chevalier A., Slama G. A fructose-rich diet decreases insulin-stimulated glucose incorporation into lipids but not glucose transport in adipocytes of normal and diabetic rats. J Nutr. 1995 Feb;125(2):164–171. doi: 10.1093/jn/125.2.164. [DOI] [PubMed] [Google Scholar]
  11. Moustaïd N., Sul H. S. Regulation of expression of the fatty acid synthase gene in 3T3-L1 cells by differentiation and triiodothyronine. J Biol Chem. 1991 Oct 5;266(28):18550–18554. [PubMed] [Google Scholar]
  12. Ntambi J. M. Dietary regulation of stearoyl-CoA desaturase 1 gene expression in mouse liver. J Biol Chem. 1992 May 25;267(15):10925–10930. [PubMed] [Google Scholar]
  13. Ntambi J. M., Sessler A. M., Takova T. A model cell line to study regulation of stearoyl-CoA desaturase gene 1 expression by insulin and polyunsaturated fatty acids. Biochem Biophys Res Commun. 1996 Mar 27;220(3):990–995. doi: 10.1006/bbrc.1996.0520. [DOI] [PubMed] [Google Scholar]
  14. Pan D. A., Hulbert A. J., Storlien L. H. Dietary fats, membrane phospholipids and obesity. J Nutr. 1994 Sep;124(9):1555–1565. doi: 10.1093/jn/124.9.1555. [DOI] [PubMed] [Google Scholar]
  15. Rubin C. S., Hirsch A., Fung C., Rosen O. M. Development of hormone receptors and hormonal responsiveness in vitro. Insulin receptors and insulin sensitivity in the preadipocyte and adipocyte forms of 3T3-L1 cells. J Biol Chem. 1978 Oct 25;253(20):7570–7578. [PubMed] [Google Scholar]
  16. Storlien L. H., Jenkins A. B., Chisholm D. J., Pascoe W. S., Khouri S., Kraegen E. W. Influence of dietary fat composition on development of insulin resistance in rats. Relationship to muscle triglyceride and omega-3 fatty acids in muscle phospholipid. Diabetes. 1991 Feb;40(2):280–289. doi: 10.2337/diab.40.2.280. [DOI] [PubMed] [Google Scholar]
  17. Waters K. M., Ntambi J. M. Insulin and dietary fructose induce stearoyl-CoA desaturase 1 gene expression of diabetic mice. J Biol Chem. 1994 Nov 4;269(44):27773–27777. [PubMed] [Google Scholar]
  18. Weiner F. R., Smith P. J., Wertheimer S., Rubin C. S. Regulation of gene expression by insulin and tumor necrosis factor alpha in 3T3-L1 cells. Modulation of the transcription of genes encoding acyl-CoA synthetase and stearoyl-CoA desaturase-1. J Biol Chem. 1991 Dec 15;266(35):23525–23528. [PubMed] [Google Scholar]

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