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. 1983 Feb;103(2):277–285. doi: 10.1093/genetics/103.2.277

Mouse Mutant Deficient in d-Amino Acid Oxidase Activity

Ryuichi Konno 1, Yosihiro Yasumura 1
PMCID: PMC1219979  PMID: 6131852

Abstract

d-Amino acid oxidase activity in the kidney homogenates of mice of seven strains was measured to search for a mutant for this enzyme. There was a consistent sex difference in the enzyme activity in these strains: male mice showed higher levels of the enzyme activity than females. In contrast to other strains, some mice of the ddY strain did not possess enzyme activity. This trait was inheritable, and a mouse stock without enzyme activity (DAO -) was established. The allele (Dao-1c) carried by the DAO- mice was recessive and behaved as a single autosomal gene in inheritance. Heterozygous mice for this gene (Dao-1 +/Dao-1c) showed nearly half the enzyme activity of the wild-type homozygotes (Dao-1+/Dao-1 +), suggesting that Dao-1c is a null allele and that there is a gene dosage effect on the enzyme activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Feinstein R. N., Howard J. B., Braun J. T., Seaholm J. E. Acatalasemic and hypocatalasemic mouse mutants. Genetics. 1966 May;53(5):923–933. doi: 10.1093/genetics/53.5.923. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fitzpatrick P. F., Massey V. Thiazolidine-2-carboxylic acid, an adduct of cysteamine and glyoxylate, as a substrate for D-amino acid oxidase. J Biol Chem. 1982 Feb 10;257(3):1166–1171. [PubMed] [Google Scholar]
  3. HESTON W. E., VLAHAKIS G., TSUBURA Y. STRAIN DD, A NEW HIGH MAMMARY TUMOR STRAIN, AND COMPARISON OF DD WITH STRAIN C3H. J Natl Cancer Inst. 1964 Jan;32:237–251. [PubMed] [Google Scholar]
  4. Hamilton G. A., Buckthal D. J., Mortensen R. M., Zerby K. W. Reactions of cysteamine and other amine metabolites with glyoxylate and oxygen catalyzed by mammalian D-amino acid oxidase. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2625–2629. doi: 10.1073/pnas.76.6.2625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Heston W. E., Hoffman H. A., Rechcigl M., Jr Genetic analysis of liver catalase activity in two substrains of C57BL mice. Genet Res. 1965 Nov;6(3):387–397. doi: 10.1017/s0016672300004274. [DOI] [PubMed] [Google Scholar]
  6. Holmes R. S. Genetics of peroxisomal enzymes in the mouse: nonlinkage of D-amino acid oxidase locus (Dao) to catalase (Cs) and L-alpha-hydroxyacid oxidase (Hao-1) loci on chromosome 2. Biochem Genet. 1976 Dec;14(11-12):981–987. doi: 10.1007/BF00485130. [DOI] [PubMed] [Google Scholar]
  7. Konno R., Uchiyama S., Yasumura Y. Intraspecies and interspecies variations in the substrate specificity of D-amino acid oxidase. Comp Biochem Physiol B. 1982;71(4):735–738. doi: 10.1016/0305-0491(82)90490-4. [DOI] [PubMed] [Google Scholar]
  8. Krebs H. A. Metabolism of amino-acids: Deamination of amino-acids. Biochem J. 1935 Jul;29(7):1620–1644. doi: 10.1042/bj0291620. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Lyle L. R., Jutila J. W. D-amino acid oxidase induction in the kidneys of germ-free mice. J Bacteriol. 1968 Sep;96(3):606–608. doi: 10.1128/jb.96.3.606-608.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. OHNISHI E., MACLEOD H., HOROWITZ N. H. Mutants of Neurospora deficient in D-amino acid oxidase. J Biol Chem. 1962 Jan;237:138–142. [PubMed] [Google Scholar]
  12. Seeley T. L., Holmes R. S. Genetics and ontogeny of butyryl CoA dehydrogenase in the mouse and linkage of Bcd-1 with Dao-1. Biochem Genet. 1981 Apr;19(3-4):333–345. doi: 10.1007/BF00504278. [DOI] [PubMed] [Google Scholar]
  13. Staats J. Standardized nomenclature for inbred strains of mice: seventh listing for the International Committee on Standardized Genetic Nomenclature for Mice. Cancer Res. 1980 Jul;40(7):2083–2128. [PubMed] [Google Scholar]
  14. VAN BEKKUM D. W., KASSENAAR A. A. The metabolic action of testosterone and related compounds. III. Effect on D-amino acid oxidase activity. Acta Endocrinol (Copenh) 1954 Jan;15(1):9–16. doi: 10.1530/acta.0.0150009. [DOI] [PubMed] [Google Scholar]
  15. Watanabe T., Motomura Y., Suga T. A new colorimetric determination of D-amino acid oxidase and urate oxidase activity. Anal Biochem. 1978 May;86(1):310–315. doi: 10.1016/0003-2697(78)90347-0. [DOI] [PubMed] [Google Scholar]
  16. de Duve D. The peroxisome: a new cytoplasmic organelle. Proc R Soc Lond B Biol Sci. 1969 Apr 15;173(1030):71–83. [PubMed] [Google Scholar]

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