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. 1992 Jul;118(7):502–508. doi: 10.1007/BF01225264

Characterization of insulin-like growth factor I receptors and growth effects in human lung cancer cell lines

Martin Rotsch 1, Michael Maasberg 1, Cebrail Erbil 1, Gabriele Jaques 1, Ursula Worsch 1, Klaus Havemann 1
PMCID: PMC12200069  PMID: 1320623

Abstract

Small-cell lung cancer (SCLC) and non-smallcell lung cancer (NSCLC) cell lines were studied for insulin-like growth factor I (IGF-I) receptor expression and with regard to the influence of IGF-I on cell proliferation. IGF-I receptors on the cells were characterized by competitive binding assays, chemical crosslinking and northern blot hybridization of IGF-I receptor mRNA. All SCLC and NSCLC cell lines showed specific IGF-I binding sites with an affinity (K D) of 0.69–5.21 nM. The amount of binding sites ranged from 59 fmol/mg to 1230 fmol/mg protein. The IGF-I binding was inhibited by the IGF-I receptor antibody (α-IR-3). Northern blot hybridization indicated that IGF-I receptor mRNA was being produced by all SCLC and NSCLC cell lines. We used the soft-agarose clonogenic assay to evaluate the influence of IGF-I on the in vitro proliferation of the cells. Our results have shown that IGF-I stimulates the growth of all tested cell lines ranging from a factor of 1.6 to 4.2 in SCLC and from 1.1 to 2.7 in NSCLC. The data indicate that the IGF-I receptor thus appears to be the common pathway for the mitogenic activity of IGF-I and IGF-II with regard to human lung cancer cells.

Key words: Small-cell lung cancer, Non-small-cell lung cancer, IGF-I receptor

Abbreviations

IGF-I

insulin-like growth factor I

SCLC

small-cell lung cancer

NSCLC

non-small-cell lung cancer

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