Abstract
Ferritin has been reported to inhibit the growth of some leukemia cells in serum-supplemented culture. Recently we have found that ferritin stimulates the proliferation of human acute myeloblastic leukemia cells HL-60 and erythroleukemia cells K-562-T1 in serum-free medium. In this study, we examined the effect of ferritin against 14 human leukemia cell lines using human heart ferritin in serum-depleted culture medium. Among 14 cell lines tested, 10 were stimulated to proliferate by ferritin (maximum response at 30–300 ng/ml) with 0–1% fetal calf serum (FCS). The growth of all the cell lines was significantly inhibited by ferritin in the presence of 10% FCS. These results suggest that ferritin has dual functions; it promotes the growth of leukemia cells with low concentrations of FCS, but suppresses their growth with high concentrations of FCS.
Key words: Ferritin, Leukemia cells, Growth promotion, Growth inhibition, Serum concentration
Abbreviations
- FCS
fetal calf serum
- SDS-PAGE
sodium dodecyl sulfate/polyacrylamide gel electrophoresis
- PBS
Dulbecco's phosphate-buffered saline
- GM-CSF
granulocyte/macrophage-colony-stimulating factor
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