Abstract
The activities ofN-acetylglucosaminyltransferase (GnT) III, IV and V were determined in 10 cases of renal cell carcinoma (RCC) and compared with the normal kidney cortex (NKC) regions of the same kidney resected from RCC patients. It was found that the GnT III and GnT IV activities decreased consistently in all samples of RCC, while GnT V activity increased, decreased or did not change in different samples. The mean levels of GnT III and GnT IV activities in RCC were found to be very significantly lower than those of NKC on statistical analysis, but the mean value of GnT V activity was almost identical in RCC and NKC. The decrease in GnT activities in RCC were compatible with the decrease in bisectingN-acetylglucosamine (GlcNAc) and antennary number of complex-typeN-glycans in γ-glutamyltranspeptidase (γ-GT) partially purified from RCCs as studied with concanavalin A (ConA) affinity column chromatography, which showed a decrease of unbound fraction and increase of bound fractions.
Key words: Renal cell carcinoma, N-acetylglucosaminyltransferase, γ-Glutamyltranspeptidase, Glycan, ConA affinity chromatography
Abbreviations
- RCC
renal cell carcinoma
- NKC
normal kidney cortex
- GnT
N-acetylglucosaminyltransferase
- γ-GT
γ-glutamyltranspeptidase
- GlcNAc
N-acetylglucosamine, Man mannose
- HPLC
high-performance liquid chromatography,N-glycan asparagine linked glycan
- EDTA
ethylenediamine tetraacetic acid
- EGTA
glycol-bis-(α-aminoethyl) etherN,N,N′,N′-tetraacetic acid
- ConA
concanavalin A
Footnotes
Supported by grants from National Natural Science Foundation of China (no 39570784) and from Ministry of Public Health
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