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. 1996 Feb;122(2):122–126. doi: 10.1007/BF01226270

Different expression patterns of cyclins A, D1 and E in human colorectal cancer

Aijin Wang 1, Naoki Yoshimi 1,✉, Masumi Suzui 1, Akiji Yamauchi 1, Makoto Tarao 1, Hideki Mori 1
PMCID: PMC12200541  PMID: 8576279

Abstract

The expressions of cyclins A, D1 and E at the protein level were investigated by Western blotting in human colorectal carcinomas and in adjacent non-neoplastic colorectal mucosas. Cyclin E was higher in the cancer tissue than in the non-neoplastic mucosa in 92% patients (35 out of 38 cases). However, the cyclin A expression of the mucosa was higher than that of the cancer tissue in 63% (25 out of 40 cases) cases, and only 4 (10%) cancers had higher cyclin A expression. Eleven cancers (27%) demonstrated expression equivalent to that in the mucosa. Equal expression of cyclin D1 in cancer and mucosal tissues was found in 51% cases (20/39), lower expression of cyclin D1 by cancer tissues was demonstrated in 41% cases (16/39) and only three cancers showed higher expression than the mucosa. Proliferating-cell nuclear antigen immunohistochemistry revealed that the labeling index of the cancer tissue was 43.5±8.3% while that of the mucosa was only 14.8±5.1%. These results proved that colorectal cancers express high levels of cyclin E, consistent with a high rate of cell proliferation, whereas most of such cancer lose control of cyclin A and cyclin D1 expression.

Key words: Cyclin A, Cyclin D1, Cyclin E, PCNA, Colorectal cancer

Abbreviations

PCNA

proliferating-cell nuclear antigen

Cdk

cyclin-dependent kinase

Cdc

cell division cycle

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