Abstract
The in vivo antitumour activity of a β1→3/1→6 glucan from the fungusGlomerella cingulata was investigated in vivo. The glucan exhibited a strong inhibition ot tumour growth of the allogeneic Sarcoma-180 as well as the syngeneic DBA/2-MC.SC-1 fibrosarcoma with inhibition ratios up to 100%. Against the hormone sensitive Noble-Nb-R prostate carcinoma the glucan alone showed a moderate antitumour effect, whereas in combination with diethylstilbestrol an almost complete regression of the tumour could be achieved. It could be demonstrated that a highly ordered structure of the glucan is not essential for the antitumour activity. Since the glucan expressed no direct cytotoxic effects, the immunomodulating activity was investigated in vitro in order to get an indication for a possible mode of action. In the lymphocyte transformation assay the glucan at a dose of 100 μg/ml caused a fourfold increase in the proliferation of murine spleen lymphocytes. Moreover, the glucan stimulated the phagocytosis of zymosan by bone marrow macrophages up to 100%. However, the glucan was not able to render macrophages cytotoxic against P-815 mastocytoma cells.
Key words: β1→3/1→6 glucan, Glomerella cingulata, Sarcoma-180, DBA/2-MC.SC-1 fibrosarcoma, Noble-Nb-R prostate carcinoma, Immunomodulation
Footnotes
The authors thank the Cerestar Research & Development, Vilvoorde (Belgium) for financial support
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