Abstract
The growth-promoting effect of several hormones and growth factors on eight human colon tumor cell lines (SW 48, SW 403, SW 480, SW 620, SW 948, HT29, LS174T and Caco-2) was studied using seven different chemically defined serum-free media [GF3: Chee's essential medium plus insulin, transferrin and selenium; GF3F: GF3 plus fetuin; GF4: GF3 plus linoleic acid/ bovine-serum albumin (BSA); GF5: GF4 plus fetuin, GF5E, GF5 plus EGF; GF5T: GF5 plus triiodothyronine; GF7: GF3 plus EGF, transferrin, insulin, linoleic acid/ BSA, oleic acid/BSA and fetuin]. GF5 appears to be the best serum-free medium as it supported continuous growth of all of the colon tumor cell lines. GF5 also supported growth of five of the seven human colon and stomach tumor xenografts as primary tissue cultures. However, the stomach xenograft cells had a very slow growth rate as compared to the colon xenograft cells in the medium. Cells grown in GF5 retained their tumorigenicity in athymic (nude) mice and characteristic cellular morphology. GF7 was the poorest of all of the serum-free media studied as none of the cell lines or xenografts grew in this medium.
Key words: Human colorectal carcinomas, Serum-free media, Tissue culture
Abbreviation
- CEM
Chee's essential medium
References
- Brower M, Carney DN, Oie HK, Gazdar AF, Minna JD (1986) Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res 46:798–806 [PubMed] [Google Scholar]
- Carney DN, Gazdar AF, Bepler G, Guccion JG, Marango PJ, Moody TW, Zweig MH, Minna JD (1985) Establishment and identification of small cell lung cancer lines having classic and variant features. Cancer Res 45:2913–2923 [PubMed] [Google Scholar]
- Grimmond HE, Hill GJ, Zirvi KA (1989) Proteins excreted by primary human colon carcinoma cells (SW 480) promote spreading and growth of metastatic human colon carcinoma cells (SW 620) in serum-free medium. Cancer Biochem. Biophys 10:353–364 [PubMed] [Google Scholar]
- Hill GJ, Zirvi KA (1988) Explantation and long-term culture of human tumors in serum-free medium. Proc Soc Surg Oncol P14, 227
- Leibovitz A, Stinson JC, McCombs WR, McCoy CE, Mazur KC, Mabry ND (1976) Classification of human colorectal adenocarcinoma cell lines. Cancer Res 36:4562–4569 [PubMed] [Google Scholar]
- McKeehan WL, Barnes D, Reid L, Stanbridge E, Murakami H, Sato GH (1990) Frontiers in mammalian cell culture. In Vitro Cell Dev Biol 26:9–23 [DOI] [PubMed] [Google Scholar]
- Shipley GD (1990) Culture of human cells in optimized media. In Vitro Cell Dev Biol 26:24A2155192 [Google Scholar]
- Spiro RG (1960) Studies of fetuin, a glycoprotein of fetal serum. J Biol Chem 235:2860–2869 [PubMed] [Google Scholar]
- van der Bosch J (1984) Primary tissue cultures of human colon carcinomas in serum-free medium: an in vitro system for tumor analysis and therapy experiments. In: Barnes DW, Sirbasku DA, Sato GH (eds) Methods of serum-free culture of epithelial and fibroblastic cells. Liss, New York, pp 73–81 [Google Scholar]
- Zirvi KA, Chee DO, Hill GJ (1986a) Continuous growth of human tumor cell lines in serum-free media. In Vitro Cell Dev Biol 22:369–374 [DOI] [PubMed] [Google Scholar]
- Zirvi KA, Dasmahapatra KS, Hill GJ (1986b) Human colorectal carcinomas grown as primary tissue cultures in serum-free media. Proc Am Assic Cancer Res 27:28 [Google Scholar]
- Zirvi KA, Golczewski JA, Hill GJ (1987a) Continuous culture of A-549 human lung carcinoma cells in serum-free medium. Proc Am Assoc Cancer Res 28:319 [Google Scholar]
- Zirvi KA, van der Bosch J, Masui H, Kaplan NO, Hill GJ (1987b) Activity of cancer chemotherapeutic agents against human colorectal carcinomas grown as primary tissue culture. J Cancer Res Clin Oncol 113:20–26 [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zirvi KA, Grimmond HE, Najjar TA (1990) Nutritional requirements for the growth of human colon tumor cells. Proc Am Assoc Dent Res 69:200 [Google Scholar]