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. 1997 Feb;123(2):82–90. doi: 10.1007/BF01269885

A new cell line from human undifferentiated carcinoma of the ovary: establishment and characterization

Jin Woo Kim 1,✉, Chun Geun Lee 2, Myung Soo Lyu 2, Heung Kee Kim 1, Jong Gu Rha 1, Dae Hoon Kim 1, Seung Jo Kim 1, Sung Eun Namkoong 1
PMCID: PMC12200780  PMID: 9030246

Abstract

A cell line designated CUMO-2 has been established from an undifferentiated ovarian carcinoma. The s.c. injection of cells into nude mice gave rise to fast-growing tumors, while the i.p. route induced a peritoneal carcinomatosis with ascites. Histopathologically, the transplanted s.c. tumors closely resembled the original tumor, but tumors developed in the peritoneal cavity were highly anaplastic. The epithelial nature of the cells was confirmed by ultrastructural analysis. Sequential cytogenetic analyses on early and late passages revealed highly aneuploid tumor cells with consistent structural aberrations of chromosomes 1, 3, 8 and 11. CUMO-2 cells were found to produce CA 125 in vitro and in vivo. Cytosol estrogen receptor (ER) was found but progesterone receptor (PR) was not measured. HLA typing indicated the presence of DR8 and DQw4. A gonadotropin-releasing hormone (Gn-RH) analog inhibited cell growth and Gn-RH receptor mRNA was detected by reverse transcription/polymerase chain reaction in this cell line. Administration of transforming growth factor β1 inhibited both cell growth and c-myc mRNA expression. This cell line demonstrated a conformational band shift in exon 7 of thep53 gene. It was a frameshift mutation.

Key Words: Human ovarian cancer, Permanent cell line, Cytogenetics, Transforming growth factor, Tumor, suppressor gene

Abbreviations

Gn-RH

gonadotropin-releasing hormone

ER

estrogen receptor

PR

progesterone receptor

RT-PCR

reverse transcription/polymerase chain reaction

TGF

transforming growth Factor

SSCP

Single-strand conformation polymorphism

MTT

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenytetrazolium bromide

Footnotes

This work was supported by a grant from the Korea research foundation, 1990

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