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. 1996 Apr;122(4):249–253. doi: 10.1007/BF01209654

Effects of okadaic acid on cell growth, anchorage-independent growth, and co-cultures of normal (KMS-6), immortalized (KMST-6), and neoplastically transformed (KMST-6T and KMST-6/RAS) human fibroblasts

Israt Jahan 1, Mikio Iijima 1, Tadashi Kondo 1, Masayoshi Namba 1,✉
PMCID: PMC12201053  PMID: 8601579

Abstract

The effects of okadaic acid (OA) on normal human (KMS-6), its immortalized (KMST-6) and neoplastically transformed (KMST-6T and KMST-6/RAS) cells were investigated as a model of two-stage carcinogenesis. The presence of OA inhibited cell growth of the normal and immortalized cells but not that of the neoplastic KMST-6T cells. In contrast, cell growth of the other neoplastic KMST-6/RAS cells transformed with the Ha-ras oncogene was inhibited by OA. OA enhanced colony formation of KMST-6T cells in soft agar, but it suppressed that of KMST-6/RAS cells. Co-cultures of KMST-6T cells with normal KMS-6 cells showed an increase in focus formation of KMST-6T cells in the presence of OA, whereas focus formation of KMST-6/RAS cells decreased. These results indicate that OA has growth-promoting effects on certain types of transformed human cells.

Key words: Tumor promoter, Okadaic acid, Human transformed cells, Anchorage-independent growth, Cell communication

Abbreviation

OA

okadaic acid

Footnotes

This work was supported by a grant-in-aid from the Ministry of Education, Science, Culture and Sports of Japan

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