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. 1991 Nov;117(6):561–567. doi: 10.1007/BF01613289

Killing of burkitt-lymphoma-derived daudi cells by ultraviolet-inactivated vaccinia virus

Leora Grunwald-Beard 1, Haim Gamliel 2,✉, Gisia Parag 1, Suresh Vedantham 2, Zichria Zakay-Rones 1
PMCID: PMC12201170  PMID: 1744162

Abstract

Interaction of active and UV-inactivated vaccinia virus at high multiplicity caused cytological changes and inhibition in cellular protein and DNA synthesis, thus arresting the multiplication of Burkitt-lymphomaderived Daudi cells and eventually killing the cells. Adsorption to the cells but the lack of penetration was evident by immunofluorescence, electron microscopy and [3H]thymidine-labeled virus incorporation. Viral DNA synthesis or virus replication was not demonstrated. Thus, it appears that the massive adsorption of viral particles, active or UV-inactivated, or possibly a “toxic” component that resides in the virion, damages the plasma membrane and may be responsible for killing the cells by a mechanism of lysis from without.

Key words: Vaccinia virus, Daudi-Burkitt lymphoma cells, Cytotoxicity, UV-inactivated virus

Abbreviations

m.o.i.

multiplicity of infection

PFU

plaque-forming units

BGM

green monkey kidney cells

References

  1. Allison AC, Sandelin K (1963) Activation of lysosomal enzymes in virus infected cells and its possible relationship to cytopathic effects. J Exp Med 117:879–887 [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bablanian R (1975) Structural and functional alterations in cultured cells infected with cytocidal viruses. Prog Med Virol 19:40–83 [PubMed] [Google Scholar]
  3. Bablanian R, Coppola G, Scribani S, Esteban M (1981) Inhibition of protein synthesis by vaccinia virus: III. the effect of ultraviolet irradiated-virus on the inhibition of protein synthesis. Virology 112:1–12 [DOI] [PubMed] [Google Scholar]
  4. Barron AI, Olshevsky C (1970) Characterization of BGM line of cells from African green monkey kidney. Arch Gesamte Virusforsch 32:382–392 [DOI] [PubMed] [Google Scholar]
  5. Bernkopf H, Nishmi M, Rosin A (1959) Effect of active and inactive virus preparations on human amnion cell cultures. J Immunol 83:635–639 [PubMed] [Google Scholar]
  6. Carrasco L (1979) Selective inhibition of protein synthesis in virus infected mammalian cells. J Virol 29:114–122 [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fitzgerald DJP, Padmanabaham R, Pastan I, Willingham MC (1983) Adeno virus induced release of epidermal growth factor and pseudomonas toxin into the cytosol of KB cells during receptor mediated endocytosis. Cell 32:607–617 [DOI] [PubMed] [Google Scholar]
  8. Gamliel H, Polliack A, Sarov I (1977) Surface features of vaccinia virus-infected human embryonic cells as studied by scanning electron microscopy. Virology 83:195–203 [DOI] [PubMed] [Google Scholar]
  9. Gamliel H (1985) Optimum fixation conditions may allow air drying of soft biological specimens with minimum cell shrinkage and maximum preservation of surface features. Scanning Electron Microsc 4:1649–1664 [PubMed] [Google Scholar]
  10. Garry RF, Bishop JM, Parker S, Westbrook K, Lewis G, Waite MRF (1979) Na+ and K-concentration and the regulation of protein synthesis in Sindbis virus infected chick cells. Virology 96:108–120 [DOI] [PubMed] [Google Scholar]
  11. Hanafusa H (1960) Killing of L cells by heat and UV inactivated vaccinia virus. Biken J 3:191–199 [Google Scholar]
  12. Hanafusa T (1960) Alteration of nucleic acid metabolism of host cells by active and inactivated forms of vaccinia virus. Biken J 3:313–327 [Google Scholar]
  13. Hanks JH, Wallace J (1958) Determination of cell viability. Proc Exp Biol Med 98:188–190 [DOI] [PubMed] [Google Scholar]
  14. Henle G, Girardi A, Henle W (1955) A non transmissible cytopathogenic effect of influenza in tissue cultures. J Exp Med 101:25–41 [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hornbeak HR, Moss B (1975) Inhibition of host protein synthesis by vaccinia virus: fate of cell M-RNA and synthesis of small poly(A)-rich polyribonucleotides in the presence of actinomycin D. J Virol 16:34–42 [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Jefferts E, Holowczak JA (1971) RNA synthesis in vaccinia infected L cells inhibition in ribosome formation and maturation. Virology 46:730–744 [DOI] [PubMed] [Google Scholar]
  17. Joklik WE (1962) The purification of four strains of poxvirus. Virology 18:9–18 [DOI] [PubMed] [Google Scholar]
  18. Klein E, Klein G, Nadkarni JS, Wigzell H, Clifford P (1968) Surface IgM-kappa specificity on Burkitt lymphoma cell in vivo and derived cultures lines. Cancer Res 28:1300–1310 [PubMed] [Google Scholar]
  19. Lacal JC, Carrasco L (1982) Relationship between membrane integrity and the inhibition of host translation in virus infected mammalian cells. Eur J Biochem 127:359–366 [DOI] [PubMed] [Google Scholar]
  20. Ledbetter MLS, Libin M (1977) Control of protein synthesis in human fibroblasts by intracellular potassium. Exp Cell Res 105:223–236 [DOI] [PubMed] [Google Scholar]
  21. Moore AE (1957) Oncolytic properties of viruses. Texas Rep Biol Med 15:588–902 [PubMed] [Google Scholar]
  22. Moss B (1968) Inhibition of HeLa cells protein synthesis by the vaccinia virus. J Virol 2:1028–1037 [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Munoz A, Castrillo JL, Carrasco L (1985) Modification of membrane permeability during Semilki Forest virus infection. Virology 146:203–212 [DOI] [PubMed] [Google Scholar]
  24. Parkhurst JR (1973) Break down of HeLa cell DNA mediated by vaccinia virus. Proc Natl Acad Sci USA 70:3200–3204 [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Pasternak CA (1987) Viruses as toxins, with special reference to Paramyxoviruses. Arch Virol 93:169–184 [DOI] [PubMed] [Google Scholar]
  26. Pedley S, Copper RJ (1984) The inhibition of HeLa cells RNA synthesis following infection with vaccinia virus. J Gen Virol 65:1687–1697 [DOI] [PubMed] [Google Scholar]
  27. Person A, Beaud G (1978) Inhibition of host protein synthesis in vaccinia virus infected cells in the presence of codycepin 3 deoxy adenosine. J Virol 25:11–18 [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Pogo BGT, Dales S (1973) Biogenesis of poxviruses, inactivation of host DNA polymerase by a component of the invading inoculum particles. Proc Natl Acad Sci USA 70:1726–1729 [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Pogo BGT, Dales S (1974) Biogenesis of poxviruses. Further evidence for inhibition of host and virus DNA synthesis by a component of the invading inoculum particles. Virology 58:377–386 [DOI] [PubMed] [Google Scholar]
  30. Polliack A, Yefenof E, Gamliel H, Klein G (1979) Burkitt's lymphoma cells: membrane properties and surface morphology as seen by scanning electron microscopy. Leukemia Res 3:217–225 [DOI] [PubMed] [Google Scholar]
  31. Rasheed S, Gottlieb AA, Garry RF (1986) Cell killing by UV inactivated human immunodeficiency virus. Virology 154:395–400 [DOI] [PubMed] [Google Scholar]
  32. Schrom M, Bablanian R (1979) Inhibition of protein synthesis by vaccinia virus. Studies on the role of virus-induced RNA synthesis. J Gen Virol 44:625–638 [DOI] [PubMed] [Google Scholar]
  33. Shlomi Y, Zakay-Rones Z (1989) Sensitivity of Burkitt lymphoma cells to inactive influenza virus. J Cancer Res Clin Oncol 115:61–66 [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Slavin S, Strober S (1978) Spontaneous murine B-cell leukemia. Nature 272:624–626 [DOI] [PubMed] [Google Scholar]
  35. Walker L (1960) In vitro cell-virus relationships resulting in cell death. Annu Rev Microbiol 14:177–196 [DOI] [PubMed] [Google Scholar]
  36. Weil-Hillman G, Zakay-Rones Z (1985) Arrest of Daudi cell growth by inactive influenza virus in vitro. Scanning Electron Microsc 4:1679–1686 [PubMed] [Google Scholar]
  37. Weil-Hillman G, Zakay-Rones Z (1987) Inhibition of Daudi tumor formation by inactive influenza virus in nude mouse model. Cancer J 1:208–212 [Google Scholar]
  38. Weil-Hillman G, Gamliel H, Friedman A, Zakay-Rones Z (1985) Visualization of the fate of inactive influenza viruses in Daudi cells in EM. Scanning Electron Microsc 4:1687–1693 [PubMed] [Google Scholar]
  39. Yaoi Y, Amano M (1970) Inhibitory effect of ultraviolet inactivated vesicular stomatitis virus on initiation of DNA synthesis in cultured chick embryo cells. J Gen Virol 9:69–75 [DOI] [PubMed] [Google Scholar]
  40. Zakay-Rones Z, Bernkopf H (1964) Effect of active and ultraviolet irradiated vaccinia virus on the development of Shay leukemia in rats. Cancer Res 24:373–378 [PubMed] [Google Scholar]
  41. Zakay-Rones Z, Rosin A, Bernkopf H (1962) A study on toxic effect of vaccinia virus in mice with special reference to hematological changes. J Immunol 88:184–190 [PubMed] [Google Scholar]

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