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. 1990 Jan;116(1):83–86. doi: 10.1007/BF01612644

Prognostic implications of DNA ploidy in squamous cell carcinomas of the tongue assessed by flow cytometry

J Hemmer 1,✉, E Schön 1, J Kreidler 1, S Haase 1
PMCID: PMC12201244  PMID: 2312608

Abstract

A total of 47 primary squamous cell carcinomas of the tongue were analysed by DNA flow cytometry. With respect to their clonal DNA content two distinct tumor groups could be distinguished. In 14 cases the tumors (29.8%) were dipoid, whereas in 33 cases (70.2%) additional cell lines characterized by abnormal DNA content could be detected. A significant increase of aneuploid cases with tumor size as well as with decreasing histological differentiation could be detected. Aneuploid cell lines are lacking in T1 as well as in most G1 carcinomas but predominate in T3 and G3. Furthermore, cervical lymph node involvement was recognized in the majority of aneuploid primary carcinomas (81.8%) but was largely lacking in diploid tumors (21.4%). Hence, the presence of aneuploid cell lines is clearly connected with clinical and histopathological parameters, each of which have turned out to worsen the prognosis in tongue carcinomas.

Key words: DNA flow cytometry, Aneuploidy, Tongue carcinoma

References

  1. Armitage NC, Robins RA, Evans DF, Turner DR, Baldwin RW, Hardcastle JD (1985) The influence of tumour cell DNA abnormalities on survival in colorectal cancer. Br J Surg 72:828–830 [DOI] [PubMed] [Google Scholar]
  2. Bijman JT, Wagener DJT, Wessels JMC, van den Broek P, Ramaekers FCS (1986) Cell size, DNA, and cytokeratin analysis of human head and neck tumors by flow cytometry. Cytometry 7:76–81 [DOI] [PubMed] [Google Scholar]
  3. Coon JS, Landay AL, Weinstein RS (1987) Biology of disease. Advances in flow cytometry for diagnostic pathology. Lab Invest 57:453–479 [PubMed] [Google Scholar]
  4. Ewers SB, Långström E, Baldetorp B, Killander D (1984) Flow cytometric DNA analysis in primary breast carcinomas and clinicopathological correlations. Cytometry 5:408–419 [DOI] [PubMed] [Google Scholar]
  5. Friedlander ML, Russel P, Talyor IW, Hedley DW, Tattersall HN (1984) Flow cytometric analysis of cellular DNA content as an adjunct to the diagnosis of ovarian tumours of borderline malignancy. Pathology 16:301–306 [DOI] [PubMed] [Google Scholar]
  6. Fries R, Grabner H, Langer H, Leijhanec J, Kränzel B, Neumann H, Schroll K, Wilfert KH (1973) Comparative investigation on the classification of carcinoma of the oral cavity. J Maxillofac Surg 1:222–235 [DOI] [PubMed] [Google Scholar]
  7. Fries R, Platz H, Wagner R, Stickler A, Grabner H, Kränzl B, Krekeler G, Kriens O, Leijhanec J, Mehnert H, Scharf F, Schroll K, Schulz P, Waldhart E, Wepner F, Zisser G (1978) Karzinome der Mundhöhle: Zur Frage der Abhängigkeit der Prognose von der Lokalisation des Primärtumors. Dtsch Z Mund Kiefer Gesch Chir 2:63–75 [Google Scholar]
  8. Goldsmith MM, Cresson DH, Arnold LA, Postma DS, Askin FB, Pillsburry HC (1987) DNA flow cytometry as a prognostic indicator in head and neck cancer. Otolaryngol Head Neck Surg 96:307–318 [DOI] [PubMed] [Google Scholar]
  9. Holm LE, Lundquist PG, Silfverswärd C, Sobin A (1982) Histological grading of malignancy in squamous cell carcinoma of the tongue. Acta Otolaryngol 94:185–192 [DOI] [PubMed] [Google Scholar]
  10. Kaplan AS, Caldarelli DD, Chacho MS, Bruce DR, Hutchinson J, Conway S, Coon JS (1986) Retrospective DNA analysis of head and neck squamous cell carcinoma. Arch Otolaryngol Head Neck Surg 112:1159–1162 [DOI] [PubMed] [Google Scholar]
  11. Kokal WA, Duda RB, Azumi N, Sheibani K, Kemeny MM, Terz JJ, Harada JR (1986) Tumor DNA content in primary and metastatic colorectal carcinoma. Arch Surg 121:1434–1439 [DOI] [PubMed] [Google Scholar]
  12. Kokal WA, Gardine RL, Sheibani K, Zak IW, Beatty JD, Riihimaki DU, Wagman LD, Terz JJ (1988) Tumor DNA content as a prognostic indicator in squamous cell carcinoma of the head and neck region. Am J Surg 156:276–280 [DOI] [PubMed] [Google Scholar]
  13. Lærum OD, Farsund T (1981) Clinical application of flow cytometry: a review. Cytometry 2:1–13 [DOI] [PubMed] [Google Scholar]
  14. Mauro F, Teodori L, Schumann J, Göhde W (1986) Flow cytometry as a tool for the prognostic assessment of human neoplasia. Int J Radiat Oncol Biol Phys 625–636 [DOI] [PubMed]
  15. Merkel DE, Dressler LG, McGuire WL (1987) Flow cytometry, cellular DNA content, and prognosis in human malignancy. J Clin Oncol 5:1690–1703 [DOI] [PubMed] [Google Scholar]
  16. Platz H, Fries R, Hudec M (1985) Retrospective DÖSAK study on carcinomas of the oral cavity: results and consequences. J Maxillofac Surg 13:147–153 [DOI] [PubMed] [Google Scholar]

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