Abstract
It was shown in the present study that irradiation of a multiclonal tumor may destroy near-diploid cell clones preferentially, while the stemline with high DNA content continues to grow after temporary arrest. This experimental observation confirms recent clinical studies.
Key words: Multiclonal tumor, Irradiation, Flow cytometry
Footnotes
Supported by a grant from Deutsche Krebshilfe
References
- Atkin NB (1971) Model DNA value and chromosome number in ovarian neoplasia. A clinical and histopathologic assessment. Cancer 27:1064–1073 [DOI] [PubMed] [Google Scholar]
- Atkin NB (1972) Modal desoxyribonucleid acid value and survival in carcinoma of the breast. Br Med J 1972/1:271–272 [DOI] [PMC free article] [PubMed] [Google Scholar]
- Atkin NB (1976a) Prognostic significance of ploidy level in human tumors: I. Carcinoma of the uterus. J Natl Cancer Inst 56:909–910 [DOI] [PubMed] [Google Scholar]
- Atkin NB (1976b) Prognostic significance of ploidy level in human tumors: II. Extra-uterine cancers and summary of data on 1171 tumors. Cytobiology 15:233–237 [PubMed] [Google Scholar]
- Atkin NB, Key R (1979) Prognostic significance of modal DNA value and other factors in malignant tumors. Br J Cancer 40:210–221 [DOI] [PMC free article] [PubMed] [Google Scholar]
- Atkin NB, Richards BM (1962) Clinical significance of ploidy in carcinoma of cervix: its relation to prognosis. Br Med J 1962/2:1445–1446 [DOI] [PMC free article] [PubMed] [Google Scholar]
- Atkin NB, Ross AJ (1960) Polyploidy in human tumors. Nature 187:579–581 [DOI] [PubMed] [Google Scholar]
- Barlogie B, Hittelmann W, Spitzer G, Hart JS, Trujillo JM, Small-wood L, Drewinko B (1977) Correlation of DNA distribution abnormalities with cytogenetic findings in human adult leukemia and lymphoma. Cancer Res 37:4400–4407 [PubMed] [Google Scholar]
- Barlogie B, Drewinko B, Schumann J, Göhde W, Dosik G, Johnston DA, Freireich EJ (1980) Cellular DNA content as a marker of neoplasia in man. Am J Med 69:195–203 [DOI] [PubMed] [Google Scholar]
- Barlogie B, Latreille J, Swartzendruber DE, Smallwood L, Maddox AM, Raber NM, Drewinko B, Alexanian R (1982) Quantitative cytometry in myeloma research. In: Schmidt WR (ed) Clinics in hematology. Saunders, Philadelphia, pp 19–46 [PubMed] [Google Scholar]
- Barlogie B, Raber NM, Schumann J, Johnson TS, Drewinko B, Swartzendruber DE, Göhde W, Andreff M, Freireich EJ (1983) Flow cytometry in clinical cancer research. Cancer Res 43:3982–3997 [PubMed] [Google Scholar]
- Brock WA, Maor MH, Peters LJ (1985) Predictors of tumor response to radiotherapy. Radiat Res [Suppl] 8:290–296 [PubMed] [Google Scholar]
- Hiddemann W, Schumann J, Andreff M, Barlogie B, Hermann CJ, Leif RC, Mayall BH, Murphy RF, Sandberg AA (1984) Convention of nomenclature for DNA cytometry. Cancer Genet Cytogenet 13:181–183 [DOI] [PubMed] [Google Scholar]
- Johnson TS, Barlogie B, Valdivieso M, Bedrossian C (1982) Ploidy and proliferative activity in human lung cancer. In: Cytometry in the clinical laboratory. Eng Foundation, Santa Barbara, Calif. [Google Scholar]
- Mellin W (1990) Cytophotometry in tumor pathology. A critical review of methods and applications, and some results of DNA analysis. Pathol Res Pract 186:37–62 [DOI] [PubMed] [Google Scholar]
- Mellin W, Dierschauer W, Hiddemann W, Roessner A, Edel G, Wuisman P, HÄrle A, Grundmann E (1989) Flow cytometric DNA analysis of bone tumors. In: Roessner A (ed) Biological characterization of bone tumors. Curr Top Pathol 80:115–152 [DOI] [PubMed]
- Olszewski W, Darzynkiewicz Z, Claps ML, Melamed MR (1982) Flow cytometry of lung carcinoma: a comparison of DNA stemline and cell cycle distribution with histology. Anal Quant Cytol 4:90–98 [PubMed] [Google Scholar]
- Thornthwaite JT, Coulson PB (1982) Comparison of steroid receptor content DNA levels, surgical staging, and survival in human breast cancer. In: Cytometry in the clinical laboratory. Eng Foundation, Santa Barbara, Calif. [Google Scholar]
- Wagner W (1989) Untersuchungen zur HeterogenitÄt und zum Strahlenresponse solider menschlicher Xerotransplantattumoren. Habilitationsschrift WWU Münster