Abstract
The in vivo localization of a polyclonal antibody (pAb) against a glycoprotein with a molecular mass of 68 kDa (GP68), which was found in developing mouse brain, was studied in murine tumor models to evaluate potential applications of this antibody for in vivo radioimmunodetection and/or therapy of cancer. The tissue distribution of125I-labeled GP68 pAb 3 days after i.V. injection into mice bearing four different kinds of solid tumor revealed a high uptake ratio by adenocarcinoma 755 and Lewis 3LL lung cancer. In contrast, the uptake ratio was low in mice bearing Ehrlich solid tumor and sarcoma-180 (S-180). These uptake ratios accorded well with the in vitro binding activity of this antibody with the tumor cells. In an immunoscintigraphic study, adenocarcinoma 755 was successfully visualized with67Ga-labeled GP68 pAb. The results of these biodistribution and in vivo radioimmunoscintigraphic studies suggest that GP68 antibody may be applicable to the diagnosis and/or therapy of cancer.
Key words: Radioimmunoimaging, Tumor, Antibody, Glycoprotein 68, Developing mouse brain
Abbreviations
- pAb
polyclonal antibody
- AFP
α-fetoprotein
- GP68
68-kDa glycoprotein
References
- Brown BA, Comeau RD, Jones PL, Liberatore FA, Neacy WP, Sands H, Gallagher BM (1987) Pharmacokinetics of the monoclonal antibody B72.3 and its fragments labeled with either125I or111In. Cancer Res 47:1149–1154 [PubMed] [Google Scholar]
- Childs RL, Hanatwich DJ (1985) Optimum conditions for labeling of DTPA-coupled antibodies with technetium-99m. J Nucl Med 26:293–299 [PubMed] [Google Scholar]
- Gold DV, Goldenberg DM (1980) Antigens associated with human solid tumor. In: Shells (ed) Cancer markers: developmental and diagnostic significance. Humana Press, Clifton NJ, pp 329–369 [Google Scholar]
- Goldenberg DM, Kim EE, Deland FH, Bennett S, Primus FJ (1980) Radioimmunodetection of cancer with radioactive antibodies to carcinoembryonic antigen. Cancer Res 40:2984–2992 [PubMed] [Google Scholar]
- Bennett S, Simmons G, Alpert E, Goldenberg DM (1980) Radioimmunodetection of cancer with radiolabeled antibodies toα-fetoprotein. Cancer Res 40:3008–3012 [PubMed] [Google Scholar]
- Koji T, Ishii N, Munehisa T, Kusumoto Y, Nakamura S, Kobayashi K, Tsukada Y, Nishi S, Hirai H (1980) Localization of radio-iodinated antibody to alpha-fetoprotein in hepatoma transplanted in rats and a case report of alpha-fetoprotein antibody treatment of a hepatoma patient. Cancer Res 40:3013–3015 [PubMed] [Google Scholar]
- Kojima S, Jay M (1987) Comparisons of labeling efficiency, biological activity and biodistribution among125I-,67Ga-DFO-, and67-Ga-DTPA-lectins. Eur J Nucl Med 13:366–370 [DOI] [PubMed] [Google Scholar]
- Nakamura K, Kubo A, Hashimoto S, Furuuchi T, Takami H, Abe O (1988) Radioimmunodetection of human pancreatic tumor xenografts using DU-PAN II monoclonal antibody. Ann Nucl Med 2:1–6 [DOI] [PubMed] [Google Scholar]
- Noguchi S, Ogata S, Sato M, Muramatsu T (1984) Stage-specific polypeptides in the developing mouse brain as demonstrated by two-dimensional gel electrophoresis. J Biochem (Tokyo) 96:881–886 [DOI] [PubMed] [Google Scholar]
- Primus FJ, Goldenberg DM (1980) Immunological considerations in the use of goat antibodies to carcinoembryonic antigen for the radioimmunodetection of cancer. Cancer Res 40:2979–2983 [PubMed] [Google Scholar]
- Sakahara H, Endo K, Nakashima T, Ohta H, Torizuka K, Okada K, Yoshida O, Hirai H (1984) Basic studies on the tumor imaging using antibodies to humanα-fetoprotein. Kaku Igaku 21:805–813 [PubMed] [Google Scholar]
- Sakahara H, Endo K, Nakashima T, Koizumi M, Ohta H, Torizuka K, Furukawa T, Ohmomo Y, Yokoyama A, Okada K, Yoshida O, Nishi S (1985) J Nucl Med 26:750–755 [PubMed] [Google Scholar]
- Sfakianakis GN, Deland FH (1982) Radioimmunodiagnosis and radioimmunotherapy. J Nucl Med 23:840–850 [PubMed] [Google Scholar]