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. 1992 Aug;118(8):601–605. doi: 10.1007/BF01211804

Frequent spontaneous sister chromatid exchange in hepatocytes of transgenic mice harboring the SV40-T antigen gene

Jia Liu 1, Hong Li 1, Kimie Nomura 1, Keiko Ohtake 1, Tomoyuki Kitagawa 1,✉
PMCID: PMC12201600  PMID: 1387648

Abstract

In order to shed light on the causal mechanisms of hepatocarcinogenesis in the transgenic mouse into which the albumin-promotor-regulated SV40-T antigen gene has been introduced (T+ mouse), and especially on the frequent chromosomal aberrations seen in cultured hepatocytes and hepatocellular neoplasms derived from such animals, the frequency of sister chromatid exchange (SCE) and karyotype abnormalities were investigated in a hepatocyte primary culture system. Cells were obtained through collagenase perfusion from T+ mice at 16–18 days of age, when no morphological changes are apparent, and from nontransgenic littermates, and cultured in the presence of bromodeoxyuridine. SCE was seen in transgenic hepatocytes twice as frequently as in their normal counterparts. No karyotype abnormalities in terms of numerical change or gross aberration were detected at this phase. The results thus suggest mutagenic properties for the T antigen, which may play an important role in hepatocarcinogenesis in this transgenic mouse.

Key words: Sister chromatid exchange, Hepatocytes, SV40-T antigen, Transgenic mice

Abbreviations

SV40

simian virus 40

T

antigen, tumor antigen

SCE

sister chromatid exchange

T

mouse, transgenic mouse in which the SV40-T antigen gene has been introduced

HCC

hepatocellular carcinoma

BrdU

bromodeoxyuridine

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