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. 1999 Aug 1;341(Pt 3):665–668.

APS, an adapter protein with a PH and SH2 domain, is a substrate for the insulin receptor kinase.

Z Ahmed 1, B J Smith 1, K Kotani 1, P Wilden 1, T S Pillay 1
PMCID: PMC1220404  PMID: 10417330

Abstract

APS (adapter protein with a PH and SH2 domain) is the newest member of a family of tyrosine kinase adapter proteins including SH2-B and Lnk. We previously identified SH2-B as an insulin-receptor-binding protein and substrate [Kotani, Wilden and Pillay (1998) Biochem J. 335, 103-109]. Here we show that APS interacts with the insulin receptor kinase activation loop through its SH2 domain and insulin stimulates the tyrosine-phosphorylation of APS. Furthermore, the phosphorylation of activation-loop tyrosine residues 1158 and 1162 are required for this interaction.

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Selected References

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