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. 1999 Sep 15;342(Pt 3):497–501.

High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells.

R R Duncan 1, A C Don-Wauchope 1, S Tapechum 1, M J Shipston 1, R H Chow 1, P Estibeiro 1
PMCID: PMC1220488  PMID: 10477258

Abstract

Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki Forest virus (SFV) vectors allow high-efficiency expression of heterologous protein in chromaffin cells.

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Selected References

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