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. 2001 Mar 15;354(Pt 3):627–633. doi: 10.1042/0264-6021:3540627

Affinity of mismatch-binding protein MutS for heteroduplexes containing different mismatches.

J Brown 1, T Brown 1, K R Fox 1
PMCID: PMC1221694  PMID: 11237867

Abstract

We have used bandshift analysis to measure the interaction between the Escherichia coli mismatch-binding protein MutS and synthetic DNA fragments containing all possible DNA mismatches as well as an unpaired T (DeltaT). The order of affinity is found to be DeltaT>GT>GG>AA approximately TT approximately TC>CA>GA>CC>GC. We find that the affinity for GT mismatches is affected by the flanking sequence and decreases in the order G(n)C(n)>(GC)(n)>A(n)T(n)>(AT)(n). Studies with base analogues show good binding to varphiT (where varphi represents 1',2'-dideoxyribose), but much weaker binding to Gvarphi.

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Selected References

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