Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 2002 Apr 1;363(Pt 1):67–72. doi: 10.1042/0264-6021:3630067

Investigation of the role of Endo180/urokinase-type plasminogen activator receptor-associated protein as a collagenase 3 (matrix metalloproteinase 13) receptor.

Louise Bailey 1, Dirk Wienke 1, Matthew Howard 1, Vera Knäuper 1, Clare M Isacke 1, Gillian Murphy 1
PMCID: PMC1222452  PMID: 11903048

Abstract

Procollagenase 3 can be activated by interaction with and cleavage by the cell-associated membrane type 1 metalloproteinase (MT1 MMP; MMP 14). It has also been shown to bind to a specific receptor, and is subsequently internalized via the low-density lipoprotein-related receptor by osteoblast cell lines. The receptor was identified as a recycling glycoprotein of the macrophage mannose receptor family, Endo180. In order to ascertain whether there is a relationship between Endo180 binding and procollagenase 3 activation, we have compared procollagenase 3 activation by an HT1080 fibrosarcoma cell line overexpressing MT1 MMP, without and with overexpression of Endo180. No difference in procollagenase 3 activation was observed, and neither was the enzyme bound to the cells or internalized. In contrast, the osteoblast cell lines, MG63 and UMR-106, both bound and internalized procollagenase 3. However, immunolocalization studies showed that the Endo180 abundantly expressed by these cells did not co-localize with the procollagenase 3. In further biochemical studies we confirmed that procollagenase 3 did not bind to Endo180, using both ligand- blotting and immunoprecipitation techniques. We conclude that Endo180 is unlikely to be a receptor for collagenase 3 in relation to either its activation or cell binding and internalization, and that other interaction partners must be sought.

Full Text

The Full Text of this article is available as a PDF (185.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkinson S. J., Patterson M. L., Butler M. J., Murphy G. Membrane type 1 matrix metalloproteinase and gelatinase A synergistically degrade type 1 collagen in a cell model. FEBS Lett. 2001 Mar 2;491(3):222–226. doi: 10.1016/s0014-5793(01)02204-9. [DOI] [PubMed] [Google Scholar]
  2. Barmina O. Y., Walling H. W., Fiacco G. J., Freije J. M., López-Otín C., Jeffrey J. J., Partridge N. C. Collagenase-3 binds to a specific receptor and requires the low density lipoprotein receptor-related protein for internalization. J Biol Chem. 1999 Oct 15;274(42):30087–30093. doi: 10.1074/jbc.274.42.30087. [DOI] [PubMed] [Google Scholar]
  3. Behrendt N., Jensen O. N., Engelholm L. H., Mørtz E., Mann M., Danø K. A urokinase receptor-associated protein with specific collagen binding properties. J Biol Chem. 2000 Jan 21;275(3):1993–2002. doi: 10.1074/jbc.275.3.1993. [DOI] [PubMed] [Google Scholar]
  4. Cowell S., Knäuper V., Stewart M. L., D'Ortho M. P., Stanton H., Hembry R. M., López-Otín C., Reynolds J. J., Murphy G. Induction of matrix metalloproteinase activation cascades based on membrane-type 1 matrix metalloproteinase: associated activation of gelatinase A, gelatinase B and collagenase 3. Biochem J. 1998 Apr 15;331(Pt 2):453–458. doi: 10.1042/bj3310453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Engelholm L. H., Nielsen B. S., Danø K., Behrendt N. The urokinase receptor associated protein (uPARAP/endo180): a novel internalization receptor connected to the plasminogen activation system. Trends Cardiovasc Med. 2001 Jan;11(1):7–13. doi: 10.1016/s1050-1738(01)00076-7. [DOI] [PubMed] [Google Scholar]
  6. Engelholm L. H., Nielsen B. S., Netzel-Arnett S., Solberg H., Chen X. D., Lopez Garcia J. M., Lopez-Otin C., Young M. F., Birkedal-Hansen H., Danø K. The urokinase plasminogen activator receptor-associated protein/endo180 is coexpressed with its interaction partners urokinase plasminogen activator receptor and matrix metalloprotease-13 during osteogenesis. Lab Invest. 2001 Oct;81(10):1403–1414. doi: 10.1038/labinvest.3780354. [DOI] [PubMed] [Google Scholar]
  7. Isacke C. M., van der Geer P., Hunter T., Trowbridge I. S. p180, a novel recycling transmembrane glycoprotein with restricted cell type expression. Mol Cell Biol. 1990 Jun;10(6):2606–2618. doi: 10.1128/mcb.10.6.2606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Knäuper V., Cowell S., Smith B., López-Otin C., O'Shea M., Morris H., Zardi L., Murphy G. The role of the C-terminal domain of human collagenase-3 (MMP-13) in the activation of procollagenase-3, substrate specificity, and tissue inhibitor of metalloproteinase interaction. J Biol Chem. 1997 Mar 21;272(12):7608–7616. doi: 10.1074/jbc.272.12.7608. [DOI] [PubMed] [Google Scholar]
  9. Knäuper V., López-Otin C., Smith B., Knight G., Murphy G. Biochemical characterization of human collagenase-3. J Biol Chem. 1996 Jan 19;271(3):1544–1550. doi: 10.1074/jbc.271.3.1544. [DOI] [PubMed] [Google Scholar]
  10. Knäuper V., Will H., López-Otin C., Smith B., Atkinson S. J., Stanton H., Hembry R. M., Murphy G. Cellular mechanisms for human procollagenase-3 (MMP-13) activation. Evidence that MT1-MMP (MMP-14) and gelatinase a (MMP-2) are able to generate active enzyme. J Biol Chem. 1996 Jul 19;271(29):17124–17131. doi: 10.1074/jbc.271.29.17124. [DOI] [PubMed] [Google Scholar]
  11. Murphy G., Stanton H., Cowell S., Butler G., Knäuper V., Atkinson S., Gavrilovic J. Mechanisms for pro matrix metalloproteinase activation. APMIS. 1999 Jan;107(1):38–44. doi: 10.1111/j.1699-0463.1999.tb01524.x. [DOI] [PubMed] [Google Scholar]
  12. Omura T. H., Noguchi A., Johanns C. A., Jeffrey J. J., Partridge N. C. Identification of a specific receptor for interstitial collagenase on osteoblastic cells. J Biol Chem. 1994 Oct 7;269(40):24994–24998. [PubMed] [Google Scholar]
  13. Sheikh H., Yarwood H., Ashworth A., Isacke C. M. Endo180, an endocytic recycling glycoprotein related to the macrophage mannose receptor is expressed on fibroblasts, endothelial cells and macrophages and functions as a lectin receptor. J Cell Sci. 2000 Mar;113(Pt 6):1021–1032. doi: 10.1242/jcs.113.6.1021. [DOI] [PubMed] [Google Scholar]
  14. Walling H. W., Chan P. T., Omura T. H., Barmina O. Y., Fiacco G. J., Jeffrey J. J., Partridge N. C. Regulation of the collagenase-3 receptor and its role in intracellular ligand processing in rat osteoblastic cells. J Cell Physiol. 1998 Dec;177(4):563–574. doi: 10.1002/(SICI)1097-4652(199812)177:4<563::AID-JCP7>3.0.CO;2-B. [DOI] [PubMed] [Google Scholar]
  15. Wu K., Yuan J., Lasky L. A. Characterization of a novel member of the macrophage mannose receptor type C lectin family. J Biol Chem. 1996 Aug 30;271(35):21323–21330. doi: 10.1074/jbc.271.35.21323. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES