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. 2002 Aug 15;366(Pt 1):79–86. doi: 10.1042/BJ20020211

Polyamine-modulated factor 1 binds to the human homologue of the 7a subunit of the Arabidopsis COP9 signalosome: implications in gene expression.

Yanlin Wang 1, Wendy Devereux 1, Tracy M Stewart 1, Robert A Casero Jr 1
PMCID: PMC1222765  PMID: 12020345

Abstract

Polyamines have been identified to play a role in the transcription of various growth-related genes. The recently discovered polyamine responsive element and the associated trans-acting proteins involved in polyamine-regulated transcription have provided a model system for the study of the role of polyamines in transcription. Polyamine-modulated factor 1 (PMF-1) was identified as one of the transacting factors that binds to NF-E2 related factor-2 (Nrf-2) to regulate the transcription of spermidine/spermine N(1)-acetyltransferase (SSAT). The possibility that PMF-1 also binds to other proteins involved in transcriptional regulation cannot be ruled out. Using a yeast two-hybrid strategy, it was found that PMF-1 binds to a human homologue of the Arabidopsis COP9 signalosome subunit 7a (CSN 7) protein. In the present study, we describe human CSN 7, a 275-amino-acid- containing protein that may have a direct role in regulating gene expression. CSN 7 and PMF-1 bind to each other, as well as compete with each other for binding to Nrf-2. This competition for Nrf-2 binding and interaction with each other is implicated in the regulation of SSAT transcription. CSN 7 possesses a C-terminal coiled-coil domain similar to the domain that mediates the interaction between PMF-1 and Nrf-2, suggesting that coiled-coil domains also mediate the interaction between CSN 7 and PMF-1. Since CSN 7 does not contain a DNA-binding domain, its effects on transcription must occur in conjunction with binding to other proteins. The results presented here demonstrate that PMF-1 and Nrf-2 can act as protein partners of CSN 7.

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Selected References

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  1. Berchtold C. M., Tamez P., Kensler T. W., Casero R. A., Jr Inhibition of cell growth in CaCO2 cells by the polyamine analogue N1,N12-bis(ethyl)spermine is preceded by a reduction in MYC oncoprotein levels. J Cell Physiol. 1998 Mar;174(3):380–386. doi: 10.1002/(SICI)1097-4652(199803)174:3<380::AID-JCP12>3.0.CO;2-D. [DOI] [PubMed] [Google Scholar]
  2. Celano P., Baylin S. B., Casero R. A., Jr Polyamines differentially modulate the transcription of growth-associated genes in human colon carcinoma cells. J Biol Chem. 1989 May 25;264(15):8922–8927. [PubMed] [Google Scholar]
  3. Celano P., Baylin S. B., Giardiello F. M., Nelkin B. D., Casero R. A., Jr Effect of polyamine depletion on c-myc expression in human colon carcinoma cells. J Biol Chem. 1988 Apr 25;263(12):5491–5494. [PubMed] [Google Scholar]
  4. Celano P., Berchtold C. M., Giardiello F. M., Casero R. A., Jr Modulation of growth gene expression by selective alteration of polyamines in human colon carcinoma cells. Biochem Biophys Res Commun. 1989 Nov 30;165(1):384–390. doi: 10.1016/0006-291x(89)91082-6. [DOI] [PubMed] [Google Scholar]
  5. Deng XW, Dubiel W, Wei N, Hofmann K, Mundt K. Unified nomenclature for the COP9 signalosome and its subunits: an essential regulator of development. Trends Genet. 2000 Jul;16(7):289–289. doi: 10.1016/s0168-9525(00)02071-0. [DOI] [PubMed] [Google Scholar]
  6. Henke W., Ferrell K., Bech-Otschir D., Seeger M., Schade R., Jungblut P., Naumann M., Dubiel W. Comparison of human COP9 signalsome and 26S proteasome lid'. Mol Biol Rep. 1999 Apr;26(1-2):29–34. doi: 10.1023/a:1006991419464. [DOI] [PubMed] [Google Scholar]
  7. Hobbs C. A., Gilmour S. K. High levels of intracellular polyamines promote histone acetyltransferase activity resulting in chromatin hyperacetylation. J Cell Biochem. 2000 Apr;77(3):345–360. [PubMed] [Google Scholar]
  8. Itoh K., Chiba T., Takahashi S., Ishii T., Igarashi K., Katoh Y., Oyake T., Hayashi N., Satoh K., Hatayama I. An Nrf2/small Maf heterodimer mediates the induction of phase II detoxifying enzyme genes through antioxidant response elements. Biochem Biophys Res Commun. 1997 Jul 18;236(2):313–322. doi: 10.1006/bbrc.1997.6943. [DOI] [PubMed] [Google Scholar]
  9. Lupas A. Coiled coils: new structures and new functions. Trends Biochem Sci. 1996 Oct;21(10):375–382. [PubMed] [Google Scholar]
  10. Lupas A. Prediction and analysis of coiled-coil structures. Methods Enzymol. 1996;266:513–525. doi: 10.1016/s0076-6879(96)66032-7. [DOI] [PubMed] [Google Scholar]
  11. Lupas A., Van Dyke M., Stock J. Predicting coiled coils from protein sequences. Science. 1991 May 24;252(5009):1162–1164. doi: 10.1126/science.252.5009.1162. [DOI] [PubMed] [Google Scholar]
  12. Patel A. R., Wang J. Y. Polyamines modulate transcription but not posttranscription of c-myc and c-jun in IEC-6 cells. Am J Physiol. 1997 Sep;273(3 Pt 1):C1020–C1029. doi: 10.1152/ajpcell.1997.273.3.C1020. [DOI] [PubMed] [Google Scholar]
  13. Seeger M., Kraft R., Ferrell K., Bech-Otschir D., Dumdey R., Schade R., Gordon C., Naumann M., Dubiel W. A novel protein complex involved in signal transduction possessing similarities to 26S proteasome subunits. FASEB J. 1998 Apr;12(6):469–478. [PubMed] [Google Scholar]
  14. Toki T., Itoh J., Kitazawa J., Arai K., Hatakeyama K., Akasaka J., Igarashi K., Nomura N., Yokoyama M., Yamamoto M. Human small Maf proteins form heterodimers with CNC family transcription factors and recognize the NF-E2 motif. Oncogene. 1997 Apr 24;14(16):1901–1910. doi: 10.1038/sj.onc.1201024. [DOI] [PubMed] [Google Scholar]
  15. Wang J. Y., Johnson L. R. Expression of protooncogenes c-fos and c-myc in healing of gastric mucosal stress ulcers. Am J Physiol. 1994 May;266(5 Pt 1):G878–G886. doi: 10.1152/ajpgi.1994.266.5.G878. [DOI] [PubMed] [Google Scholar]
  16. Wang J. Y., McCormack S. A., Viar M. J., Wang H., Tzen C. Y., Scott R. E., Johnson L. R. Decreased expression of protooncogenes c-fos, c-myc, and c-jun following polyamine depletion in IEC-6 cells. Am J Physiol. 1993 Aug;265(2 Pt 1):G331–G338. doi: 10.1152/ajpgi.1993.265.2.G331. [DOI] [PubMed] [Google Scholar]
  17. Wang Y., Devereux W., Stewart T. M., Casero R. A., Jr Cloning and characterization of human polyamine-modulated factor-1, a transcriptional cofactor that regulates the transcription of the spermidine/spermine N(1)-acetyltransferase gene. J Biol Chem. 1999 Jul 30;274(31):22095–22101. doi: 10.1074/jbc.274.31.22095. [DOI] [PubMed] [Google Scholar]
  18. Wang Y., Xiao L., Thiagalingam A., Nelkin B. D., Casero R. A., Jr The identification of a cis-element and a trans-acting factor involved in the response to polyamines and polyamine analogues in the regulation of the human spermidine/spermine N1-acetyltransferase gene transcription. J Biol Chem. 1998 Dec 18;273(51):34623–34630. doi: 10.1074/jbc.273.51.34623. [DOI] [PubMed] [Google Scholar]
  19. Wei N., Chamovitz D. A., Deng X. W. Arabidopsis COP9 is a component of a novel signaling complex mediating light control of development. Cell. 1994 Jul 15;78(1):117–124. doi: 10.1016/0092-8674(94)90578-9. [DOI] [PubMed] [Google Scholar]
  20. Wei N., Deng X. W. COP9: a new genetic locus involved in light-regulated development and gene expression in arabidopsis. Plant Cell. 1992 Dec;4(12):1507–1518. doi: 10.1105/tpc.4.12.1507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wei N., Deng X. W. Characterization and purification of the mammalian COP9 complex, a conserved nuclear regulator initially identified as a repressor of photomorphogenesis in higher plants. Photochem Photobiol. 1998 Aug;68(2):237–241. doi: 10.1562/0031-8655(1998)068<0237:capotm>2.3.co;2. [DOI] [PubMed] [Google Scholar]
  22. Wei N., Tsuge T., Serino G., Dohmae N., Takio K., Matsui M., Deng X. W. The COP9 complex is conserved between plants and mammals and is related to the 26S proteasome regulatory complex. 1998 Jul 30-Aug 13Curr Biol. 8(16):919–922. doi: 10.1016/s0960-9822(07)00372-7. [DOI] [PubMed] [Google Scholar]
  23. Yahalom A., Kim T. H., Winter E., Karniol B., von Arnim A. G., Chamovitz D. A. Arabidopsis eIF3e (INT-6) associates with both eIF3c and the COP9 signalosome subunit CSN7. J Biol Chem. 2001 Jan 5;276(1):334–340. doi: 10.1074/jbc.M006721200. [DOI] [PubMed] [Google Scholar]

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