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. 2002 Oct 1;367(Pt 1):247–254. doi: 10.1042/BJ20020393

Rapid insertion of GLUT2 into the rat jejunal brush-border membrane promoted by glucagon-like peptide 2.

Anita Au 1, Alina Gupta 1, Paul Schembri 1, Chris I Cheeseman 1
PMCID: PMC1222871  PMID: 12095416

Abstract

A possible role for GLUT2 transiently expressed in the rat jejunal brush-border membrane (BBM) under the influence of glucagon-like peptide 2 (GLP-2) was investigated using in vivo perfusion of the intestinal lumen as well as isolation of membrane proteins and immunohistochemistry. A 1 h vascular infusion of GLP-2 in vivo doubled the rate of fructose absorption and this increase could be blocked by luminal phloretin. Immunohistochemistry of frozen sections of rat jejunum showed the expression of GLUT2 in both the basolateral and BBMs of mature enterocytes. Perfusion of the intestinal lumen with 50 mM D-glucose or vascular infusion of 800 pM GLP-2 for 1 h increased the expression of GLUT2 in the BBM. Quantification of these changes using Western blotting of biotinylated surface-exposed protein showed a doubling of the expression of GLUT2 in the BBM, but the effects of glucose and GLP-2 were not additive. These results indicate that vascular GLP-2 can promote the insertion of GLUT2 into the rat jejunal BBM providing a low-affinity/high-capacity route of entry for dietary hexoses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alvarado F. Hypothesis for the interaction of phlorizin and phloretin with membrane carriers for sugars. Biochim Biophys Acta. 1967 Jul 3;135(3):483–495. doi: 10.1016/0005-2736(67)90038-7. [DOI] [PubMed] [Google Scholar]
  2. Cheeseman C. I., O'Neill D. Basolateral D-glucose transport activity along the crypt-villus axis in rat jejunum and upregulation induced by gastric inhibitory peptide and glucagon-like peptide-2. Exp Physiol. 1998 Sep;83(5):605–616. doi: 10.1113/expphysiol.1998.sp004142. [DOI] [PubMed] [Google Scholar]
  3. Cheeseman C. I., Tsang R. The effect of GIP and glucagon-like peptides on intestinal basolateral membrane hexose transport. Am J Physiol. 1996 Sep;271(3 Pt 1):G477–G482. doi: 10.1152/ajpgi.1996.271.3.G477. [DOI] [PubMed] [Google Scholar]
  4. Cheeseman C. I. Upregulation of SGLT-1 transport activity in rat jejunum induced by GLP-2 infusion in vivo. Am J Physiol. 1997 Dec;273(6 Pt 2):R1965–R1971. doi: 10.1152/ajpregu.1997.273.6.R1965. [DOI] [PubMed] [Google Scholar]
  5. Corpe C. P., Basaleh M. M., Affleck J., Gould G., Jess T. J., Kellett G. L. The regulation of GLUT5 and GLUT2 activity in the adaptation of intestinal brush-border fructose transport in diabetes. Pflugers Arch. 1996 Jun;432(2):192–201. doi: 10.1007/s004240050124. [DOI] [PubMed] [Google Scholar]
  6. Crouzoulon G., Korieh A. Fructose transport by rat intestinal brush border membrane vesicles. Effect of high fructose diet followed by return to standard diet. Comp Biochem Physiol A Comp Physiol. 1991;100(1):175–182. doi: 10.1016/0300-9629(91)90203-o. [DOI] [PubMed] [Google Scholar]
  7. Crouzoulon G., Korieh A. Fructose transport by rat intestinal brush border membrane vesicles. Effect of high fructose diet followed by return to standard diet. Comp Biochem Physiol A Comp Physiol. 1991;100(1):175–182. doi: 10.1016/0300-9629(91)90203-o. [DOI] [PubMed] [Google Scholar]
  8. Davidson N. O., Hausman A. M., Ifkovits C. A., Buse J. B., Gould G. W., Burant C. F., Bell G. I. Human intestinal glucose transporter expression and localization of GLUT5. Am J Physiol. 1992 Mar;262(3 Pt 1):C795–C800. doi: 10.1152/ajpcell.1992.262.3.C795. [DOI] [PubMed] [Google Scholar]
  9. Helliwell P. A., Richardson M., Affleck J., Kellett G. L. Regulation of GLUT5, GLUT2 and intestinal brush-border fructose absorption by the extracellular signal-regulated kinase, p38 mitogen-activated kinase and phosphatidylinositol 3-kinase intracellular signalling pathways: implications for adaptation to diabetes. Biochem J. 2000 Aug 15;350(Pt 1):163–169. [PMC free article] [PubMed] [Google Scholar]
  10. Helliwell P. A., Richardson M., Affleck J., Kellett G. L. Stimulation of fructose transport across the intestinal brush-border membrane by PMA is mediated by GLUT2 and dynamically regulated by protein kinase C. Biochem J. 2000 Aug 15;350(Pt 1):149–154. [PMC free article] [PubMed] [Google Scholar]
  11. Hirsh A. J., Tsang R., Kammila S., Cheeseman C. I. Effect of cholecystokinin and related peptides on jejunal transepithelial hexose transport in the Sprague-Dawley rat. Am J Physiol. 1996 Nov;271(5 Pt 1):G755–G761. doi: 10.1152/ajpgi.1996.271.5.G755. [DOI] [PubMed] [Google Scholar]
  12. Ikeda T. S., Hwang E. S., Coady M. J., Hirayama B. A., Hediger M. A., Wright E. M. Characterization of a Na+/glucose cotransporter cloned from rabbit small intestine. J Membr Biol. 1989 Aug;110(1):87–95. doi: 10.1007/BF01870995. [DOI] [PubMed] [Google Scholar]
  13. Ikeda T. S., Hwang E. S., Coady M. J., Hirayama B. A., Hediger M. A., Wright E. M. Characterization of a Na+/glucose cotransporter cloned from rabbit small intestine. J Membr Biol. 1989 Aug;110(1):87–95. doi: 10.1007/BF01870995. [DOI] [PubMed] [Google Scholar]
  14. Inukai K., Katagiri H., Takata K., Asano T., Anai M., Ishihara H., Nakazaki M., Kikuchi M., Yazaki Y., Oka Y. Characterization of rat GLUT5 and functional analysis of chimeric proteins of GLUT1 glucose transporter and GLUT5 fructose transporter. Endocrinology. 1995 Nov;136(11):4850–4857. doi: 10.1210/endo.136.11.7588216. [DOI] [PubMed] [Google Scholar]
  15. Jordan N. J., Holman G. D. Photolabelling of the liver-type glucose-transporter isoform GLUT2 with an azitrifluoroethylbenzoyl-substituted bis-D-mannose. Biochem J. 1992 Sep 1;286(Pt 2):649–656. doi: 10.1042/bj2860649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kane S., Seatter M. J., Gould G. W. Functional studies of human GLUT5: effect of pH on substrate selection and an analysis of substrate interactions. Biochem Biophys Res Commun. 1997 Sep 18;238(2):503–505. doi: 10.1006/bbrc.1997.7204. [DOI] [PubMed] [Google Scholar]
  17. Kellett G. L., Helliwell P. A. The diffusive component of intestinal glucose absorption is mediated by the glucose-induced recruitment of GLUT2 to the brush-border membrane. Biochem J. 2000 Aug 15;350(Pt 1):155–162. [PMC free article] [PubMed] [Google Scholar]
  18. Kellett G. L. The facilitated component of intestinal glucose absorption. J Physiol. 2001 Mar 15;531(Pt 3):585–595. doi: 10.1111/j.1469-7793.2001.0585h.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Limb C., Tamborlane W. V., Sherwin R. S., Pederson R., Caprio S. Acute incretin response to oral glucose is associated with stimulation of gastric inhibitory polypeptide, not glucagon-like peptide in young subjects. Pediatr Res. 1997 Mar;41(3):364–367. doi: 10.1203/00006450-199703000-00010. [DOI] [PubMed] [Google Scholar]
  20. Madara J. L., Pappenheimer J. R. Structural basis for physiological regulation of paracellular pathways in intestinal epithelia. J Membr Biol. 1987;100(2):149–164. doi: 10.1007/BF02209147. [DOI] [PubMed] [Google Scholar]
  21. Maenz D. D., Cheeseman C. I. Effect of hyperglycemia on D-glucose transport across the brush-border and basolateral membrane of rat small intestine. Biochim Biophys Acta. 1986 Aug 21;860(2):277–285. doi: 10.1016/0005-2736(86)90524-9. [DOI] [PubMed] [Google Scholar]
  22. Martín M. G., Lostao M. P., Turk E., Lam J., Kreman M., Wright E. M. Compound missense mutations in the sodium/D-glucose cotransporter result in trafficking defects. Gastroenterology. 1997 Apr;112(4):1206–1212. doi: 10.1016/s0016-5085(97)70132-x. [DOI] [PubMed] [Google Scholar]
  23. Miyamoto K., Tatsumi S., Morimoto A., Minami H., Yamamoto H., Sone K., Taketani Y., Nakabou Y., Oka T., Takeda E. Characterization of the rabbit intestinal fructose transporter (GLUT5). Biochem J. 1994 Nov 1;303(Pt 3):877–883. doi: 10.1042/bj3030877. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Miyamoto K., Tatsumi S., Morimoto A., Minami H., Yamamoto H., Sone K., Taketani Y., Nakabou Y., Oka T., Takeda E. Characterization of the rabbit intestinal fructose transporter (GLUT5). Biochem J. 1994 Nov 1;303(Pt 3):877–883. doi: 10.1042/bj3030877. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Pappenheimer J. R. Physiological regulation of epithelial junctions in intestinal epithelia. Acta Physiol Scand Suppl. 1988;571:43–51. [PubMed] [Google Scholar]
  26. Rand E. B., Depaoli A. M., Davidson N. O., Bell G. I., Burant C. F. Sequence, tissue distribution, and functional characterization of the rat fructose transporter GLUT5. Am J Physiol. 1993 Jun;264(6 Pt 1):G1169–G1176. doi: 10.1152/ajpgi.1993.264.6.G1169. [DOI] [PubMed] [Google Scholar]
  27. Thorens B., Cheng Z. Q., Brown D., Lodish H. F. Liver glucose transporter: a basolateral protein in hepatocytes and intestine and kidney cells. Am J Physiol. 1990 Dec;259(6 Pt 1):C279–C285. doi: 10.1152/ajpcell.1990.259.2.C279. [DOI] [PubMed] [Google Scholar]

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