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. 2003 Mar 15;370(Pt 3):763–769. doi: 10.1042/BJ20021545

Generation of hepatocytes expressing functional cytochromes P450 from a pancreatic progenitor cell line in vitro.

Carylyn J Marek 1, Gary A Cameron 1, Lucy J Elrick 1, Gabrielle M Hawksworth 1, Matthew C Wright 1
PMCID: PMC1223247  PMID: 12542397

Abstract

The proliferating AR42J-B13 pancreatic cell line is known to respond to glucocorticoid treatment by producing foci of cells that express the liver-specific albumin gene. We demonstrate that this cell line also expresses liver-specific or liver-enriched functional cytochrome P450 proteins when stimulated to trans-differentiate into hepatocytes by glucocorticoid. These data suggest that this cell line has an unusual ability to trans-differentiate into functional hepatocytes and that it could be possible to generate a limitless supply of functional hepatocyte-like cells in vitro.

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Selected References

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