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. 2004 Apr 1;379(Pt 1):125–131. doi: 10.1042/BJ20031485

Beta-glucosidase, exo-beta-glucanase and pyridoxine transglucosylase activities of rice BGlu1.

Rodjana Opassiri 1, Yanling Hua 1, Onnop Wara-Aswapati 1, Takashi Akiyama 1, Jisnuson Svasti 1, Asim Esen 1, James R Ketudat Cairns 1
PMCID: PMC1224054  PMID: 14692878

Abstract

The bglu1 cDNA for a beta-glucosidase cloned from rice (Oryza sativa L.) seedlings was expressed as a soluble and active protein in Escherichia coli and designated BGlu1. This enzyme hydrolysed beta-1,4-linked oligosaccharides with increasing catalytic efficiency (kcat/Km) values as the DP (degree of polymerization) increased from 2 to 6. In contrast, hydrolysis of beta-1,3-linked oligosaccharides decreased from DP 2 to 3, and polymers with a DP greater than 3 were not hydrolysed. The enzyme also hydrolysed p -nitrophenyl beta-D-glycosides and some natural glucosides but with lower catalytic efficiency than beta-linked oligosaccharides. Pyridoxine 5'-O-beta-D-glucoside was the most efficiently hydrolysed natural glycoside tested. BGlu1 also had high transglucosylation activity towards pyridoxine, producing pyridoxine 5'-O-beta-D-glucopyranoside in the presence of the glucose donor p-nitrophenyl beta-D-glucoside.

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Selected References

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