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. 1988 Dec;114(6):565–574. doi: 10.1007/BF00398178

Histiocytic differentiation in benign and malignant bone tumors

Andreas Meyer 1, Tillmann Steinmeier 1, Thomas Löning 1,✉, Heinz Joachim Radzun 2, Günter Delling 1
PMCID: PMC12243733  PMID: 2462563

Abstract

In this study fresh frozen tissue samples of benign osseous tumors (five non-osteogenic fibromas, one fibrous dysplasia, one chondromyxoidfibroma), tumors of uncertain biological behaviour (eight cases of histiocytosis X, two giant-cell tumors), and of malignant intraosseous tumors (two malignant fibrous histiocytomas, two malignant histiocytosis, four osteosarcomas, one chondrosarcoma and two Ewing sarcomas) were studied with a panel of monoclonal antibodies reactive with monocyte/macrophages and various types of dendritic cells. In addition, tumors were further defined with a broad spectrum of antibodies against filamentous proteins and lymphocyte differentiation antigens. The specimens were stained with a triple-layer immunoalkaline phosphatase protocol. Tumors stained with these antibodies could be roughly divided into two groups. The first group comprised tumors with one predominant cell population reactive with one particular monoclonal antibody. In this group, cases of histiocytosis X were found to be consistently labelled with CD-1 antibodies. The giantcell tumors showed a very homogeneous staining with certain monocyte/macrophage antibodies (Ki-M8). Nevertheless, even in these tumors, heterogeneity was demonstrated by the occurrence of cells with monocytic differentiation in histiocytosis X and conversely by the occurrence of cells with differentiation antigens of the dendritic cell system in giant-cell tumors. An exception has to be made for the two cases of malignant histocytosis examined. These tumors were selectively labelled with antibodies against monocyte/macrophages (Ki-M8, IOM-1). The second group comprised tumors showing a high degree of heterogeneity demonstrated by the varying amounts of tumor cells reacting with the applied markers of the monocyte/macrophage and dendritic cell systems. In most cases it was difficult to ascribe labelled cells to the tumor cell population as opposed to an “innocent bystander” inflammatory cell population. This distinction was especially difficult in malignant fibrous histiocytomas underlining the current concept that these tumors are of primitive mesenchymal rather than true histiocytic origin.

Key words: Monoclonal antibodies, Immunohistochemistry, Macrophage/monocyte antigen, Fibrohistiocytic bone tumors

Footnotes

This study was supported by Deutsche Forschungsgemeinschaft and Hamburger Stiftung zur Förderung der Krebsbekämpfung

References

  1. Aozasa K, Tsujimoto M, Inoue A (1985) Malignant histiocytosis. Report of twenty-five cases with pulmonary, renal and/or gastro-intestinal involvement. Histopathology 9:39–49 [DOI] [PubMed] [Google Scholar]
  2. Athanasou NA, Bliss E, Gatter KC, Heryet A (1985) An immunohistological study of giant-cell tumor of bone: evidence for an osteoclast origin of the giant cells. J Pathol 147:153–158 [DOI] [PubMed] [Google Scholar]
  3. Brecher ME, Franklin WA, Simon MA (1986) Immunohistochemical study of mononuclear phagocyte antigens in giant cell tumor of bone. Am J Pathol 125:252–257 [PMC free article] [PubMed] [Google Scholar]
  4. Burckhardt A (1987) Knochentumoren im Kiefer- und Gesichstsbereich. Pathologe 8:9–19 [PubMed] [Google Scholar]
  5. Cordell JL, Falini B, Erber WN, Ghosh AK, Abdulaziz A, MacDonald S, Pulford KAF, Stein H, Mason DY (1984) Immunoenzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP). J Histochem Cytochem 32:219–229 [DOI] [PubMed] [Google Scholar]
  6. Cunningham JB, Ackermann LV (1956) Metaphyseal fibrous defects. J Bone Joint Surg [Am] 38-A:797–808 [PubMed] [Google Scholar]
  7. Dahlin DC (1977) Bone tumors, general aspects and data on 6221 cases. 3rd edn, Charles C. Thomas, Springfield/Ill. [Google Scholar]
  8. Dana N, Todd III RF, Pitt J, Springer JA, Arnaout MA (1984) Deliciency of a surface membrane glycoprotein (Mo1) in man. J Clin Invest 73:153–159 [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Delling G (1984) Skelettsystem. In: Remmele W (ed) Pathologie, Bd 3. Springer, Berlin Heidelberg New York, pp 639–740 [Google Scholar]
  10. Ducatman BS, Wick MR, Morgan TW, Banks PM, Pierre RV (1984) Malignant histiocytosis. Hum Pathol 15:368–377 [DOI] [PubMed] [Google Scholar]
  11. Enzinger M, Weiss SM (1983) Soft tissue tumors. The C.V. Mosby Company, St. Louis [Google Scholar]
  12. Esseltine DW, De Leeuw KM, Berry GR (1983) Malignant histiocytosis. Cancer 52:1904–1910 [DOI] [PubMed] [Google Scholar]
  13. Franklin WA, Mason DY, Pulford K (1986) Immunohistological analysis of human mononuclear phagocytes and dendritic cells by using monoclonal antibodies. Lab Invest 54:322–335 [PubMed] [Google Scholar]
  14. Isaacson PG (1985) Histiocytic malignancy. Histopathology 9:1007–1011 [DOI] [PubMed] [Google Scholar]
  15. Koizumi S, Yamagami M, Miura M, Horita S, Sano M, Ikuta N, Taniguchi N (1982) Expression of Ia-like antigens defined by monoclonal OKIa1 antibody on hemopoietic progenitor cells in cord blood: a comparison with human bone marrow. Blood 60:1046–1049 [PubMed] [Google Scholar]
  16. Kreipe H, Radzun HJ, Parwaresch MR, Haislip A, Hansmann M-L (1987) Ki-M7 monoclonal antibody specific for myelomonocytic cell lineage and macrophages in human. J Histochem Cytochem 35:1117–1126 [DOI] [PubMed] [Google Scholar]
  17. Lawson CW, Fisher C, Gatter KC (1987) An immunohistochemical study of differentiation in malignant fibrous histiocytoma. Histophatology 11:375–383 [DOI] [PubMed] [Google Scholar]
  18. McMillian EM, Humphrey GB, Stoneking L, Strauss L, Civin CI, Abo T, Balch C (1986) Analysis of histiocytosis X infiltrates with monoclonal antibodies directed against cells of histiocytic, lymphoid, and myeloid lineage. Clin Immunol Immunopathol 38:295–301 [DOI] [PubMed] [Google Scholar]
  19. Mellin W, Roessner A, Grundmann E, Wörmann B, Hiddemann W, Immenkamp M (1985) Blological characterization of human bone tumors. VII. Detection of malignancy in a giant-cell tumor of bone by flow cytometric DNA-analysis. Pathol Res Pract 180:619–625 [DOI] [PubMed] [Google Scholar]
  20. Parwaresch MR, Radzun HJ, Hansmann M-L, Peters K-L, (1983) Monoclonal antibody Ki-M4 specifically recognizes human dendritic reticulum cells (follicular dendritic cells) and their possible precursor in blood. Blood 62:585–590 [PubMed] [Google Scholar]
  21. Parwaresch MR, Radzun HJ, Kreipe H, Hansmann M-L, Barth J (1986) Monocyte/macrophage-reactive monoclonal antibody Ki-M6 recognizes an intracytoplasmic antigen. Am J Pathol 125:141–151 [PMC free article] [PubMed] [Google Scholar]
  22. Radzun HJ, Parwaresch MR (1983) Differential immunohistochemical resolution of the human mononuclear phagocyte system. Cell Immunol 82:174–183 [DOI] [PubMed] [Google Scholar]
  23. Radzun HJ, Parwaresch MR, Feller AC, Hansmann M-L (1984) Monocyte/macrophage-specific monoclonal antibody Ki-M1 recognizes interdigitating reticulum cells. Am J Pathol 117:441–450 [PMC free article] [PubMed] [Google Scholar]
  24. Radzun HJ, Kreipe H, Bödewadt S, Hansmann M-L, Barth J, Parwaresch MR (1987) Ki-M8 monoclonal antibody reactive with an intracytoplasmic antigen of monocyte/macrophage lineage. Blood 69:1320–1327 [PubMed] [Google Scholar]
  25. Rahimi A, Beabout JW, Ivins JC, Dahlin DC (1972) Chondromyxoid fibroma: a clinicopathologic study of 76 cases. Cancer 30:726–730 [DOI] [PubMed] [Google Scholar]
  26. Ralph LL (1962) Chondromyxoid fibroma of bone. J Bone Joint Surg [Br] 44B:7–24 [DOI] [PubMed] [Google Scholar]
  27. Reinherz EL, Kung PC, Goldstein G, Levey RH, Schlossmann SF (1980) Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage. Proc Natl Acad Sci USA 77:1588–1592 [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Roessner A, v Bassewitz DB, Schlake W, Thorwesten G, Grundmann E (1984) Biologic characterization of human bone tumors. III. Giant cell tumor of bone a combined electron microscopical, histochemical, and autoradiographical study. Pathol Res Pract 178:432–440 [DOI] [PubMed] [Google Scholar]
  29. Roessner A, Vassallo J, Vollmer E, Zwadlo G, Sorg C, Grundmann E (1987a) Biological characterization of human bone tumors. X. The proliferation behavior of macrophages as compared to fibroblastic cells in malignant fibrous histiocytoma and giant cell tumor. J Cancer Res Clin Oncol 113:559–565 [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Roessner A, Zwadlo G, Vollmer E, Sorg C, Grundmann E (1987b) Biologic characterization of human bone tumors. IX. Occurrence of macrophages. Pathol Res Pract 182:336–343 [DOI] [PubMed] [Google Scholar]
  31. Roholl PJM, Kleyne J, Elbers H, Van der Veigt MCD, Albus-Lutter CHE, van Unnik JAM (1985) Characterization of tumor cells in malignant fibrous histiocytoma and other soft tissue tumors in comparison with malignant histiocytes. I. Immunohistochemical study on paraffin sections. J Pathol 147:87–95 [DOI] [PubMed] [Google Scholar]
  32. Rosseau-Merck MF, Barbey S, Jaubert F, Bach MA, Chatenoud L, Nezelof C (1983) Reactivity of histiocytosis X cells with monoclonal antibodies. Pathol Rest Pract 177:8–12 [DOI] [PubMed] [Google Scholar]
  33. Salzer M, Salzer-Kuntschick M (1965) Das Chondromyxoidfibrom. Langenbecks. Arch Klin Chir 312:216–231 [DOI] [PubMed] [Google Scholar]
  34. Salzer-Kuntschick M, Brand M, Delling G (1983) Bestimmung des morphologischen Regressionsgrades nach Chemotherapie bei malignen Knochentumoren. Pathologe 4:135–221 [PubMed] [Google Scholar]
  35. Schajowitz F, Ackermann LV, Sissons HA (1972) Histologic typing of bone tumors. International histological classification of tumors, no. 6. World Health Organisation, Geneva [Google Scholar]
  36. Stein H, Gatter K, Asbahr H, Mason DY (1985) Methods in laboratory investigation. Lab Invest 52:676–683 [PubMed] [Google Scholar]
  37. Strauchen JA, Dimitri-Bona A (1986) Malignant fibrous histiocytoma. Expression of monocyte/macrophage differentiation antigens detected with monoclonal antibodies. Am J Pathol 124:303–309 [PMC free article] [PubMed] [Google Scholar]
  38. Tagawa S, Konishi I, Kuratune H, Katagiri S, Taniguchi N, Tamaki T, Inoue R, Kanayama Y, Tsubakio T, Machii T (1983) A case of T-cell chronic lymphocytic leukemia (T-CLL) expressing a peculiar phenotype (E+, OKM1+, Leu1+, OKT3- and 3gGEA-). Cancer 52:1378–1384 [DOI] [PubMed] [Google Scholar]
  39. Turner RR, Wood GS, Beckstead JH, Colgy TV, Horning SJ, Warnke RA (1984) Histiocytic malignancies. Am J Surg Pathol 8:485–500 [PubMed] [Google Scholar]
  40. Weiss LM, Azzi R, Dorfman RF, Warnke RA (1986) Sinusoidal hematolymphoid malignancy (“malignant histiocytosis”) presenting as atypical proliferation. Cancer 58:1681–1688 [DOI] [PubMed] [Google Scholar]
  41. Wood GS, Beckstead JH, Turner RR, Hendrickson MR, Kempson RL, Warnke RA (1986) Malignant fibrous histiocytoma tumor cells resemble fibroblasts. Am J Surg Pathol 10(5):323–335 [DOI] [PubMed] [Google Scholar]
  42. Yonish-Rouach E, Viac J, Schmitt D, Cordier G, Thivolet J, Brochier J (1982) Preparation of monoclonal antihuman thymocyte antibodies; unexpected markers of epidermal cells. In: Serrou B (ed) Current concepts in human immunology and cancer immunotherapy, vol 17. Biomedical Press p 55

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