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. 1988 Aug 1;114(4):373–379. doi: 10.1007/BF02128181

Analysis of metastatic competence of mouse bladder carcinoma cells after transfection with activated Ha-ras or N-ras oncogenes

J Pohl 1, A Radler-Pohl 1, L M Franks 4, V Schirrmacher 1,✉
PMCID: PMC12243773  PMID: 3410877

Abstract

Transfection of the Ha-ras oncogene into a low metastatic epithelial cell line resulted in the acquirement of significantly increased metastatic capacity. This alteration in metastatic competence of a carcinoma line in a syngeneic system seemed to be a selective change and was not affected by parameters such as tumor latency period or local tumor growth. Transfection of the selection marker vectors with normal cellular DNA or with the N-ras gene did not lead to significantly increased metastatic capacity. Analysis of metastatic variants after oncogene transfection and in vivo selection showed integration of N-ras, but not of Ha-ras oncogenes. A possible role for the Ha-ras oncogene in the initial steps of metastasis will be discussed.

Key words: Oncogene, Ras, Metastasis, Transfection

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