Abstract
An Hfr13 ΔproA-lac deletion recipient, -ΔproA-lac-F-purE+-, has been utilized in a study of the origins of duplications formed during chromosome fragment integration. Among the Pro-Lac+ transductants, some have duplications spanning the F locus. These transductants are, or segregate, strains with F' episomes carrying genes of the duplication. Some of the duplications include purE+, a gene which is not coinherited with lac+ during bacteriophage P1-mediated transduction. Thus recipient genes have been duplicated during recombinant formation. Crossing-over models including replication steps provide a basis for explaining the duplication process.
Full Text
The Full Text of this article is available as a PDF (905.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- MARKOVITZ A. REGULATORY MECHANISMS FOR SYNTHESIS OF CAPSULAR POLYSACCHARIDE IN MUCOID MUTANTS OF ESCHERICHIA COLI K12. Proc Natl Acad Sci U S A. 1964 Feb;51:239–246. doi: 10.1073/pnas.51.2.239. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sgaramella V., Van de Sande J. H., Khorana H. G. Studies on polynucleotides, C. A novel joining reaction catalyzed by the T4-polynucleotide ligase. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1468–1475. doi: 10.1073/pnas.67.3.1468. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stadler D. R., Towe A. M. Evidence for meiotic recombination in Ascobolus involving only one member of a tetrad. Genetics. 1971 Jul;68(3):401–413. doi: 10.1093/genetics/68.3.401. [DOI] [PMC free article] [PubMed] [Google Scholar]