Abstract
Experiments are described that provide an opportunity to estimate the genetic limits of the structural (amino acid coding) portion of the rosy locus (3:52.0) in Drosophila melanogaster, which controls the enzyme, xanthine dehydrogenase (XDH). This is accomplished by mapping experiments which localize sites responsible for electrophoretic variation in the enzyme on the known genetic map of null-XDH rosy mutants. Electrophoretic sites are distributed along a large portion of the null mutant map. A cis-trans test involving electrophoretic variants in the left- and right-hand portions of the map leads to the conclusion that the entire region between these variants is also structural. Hence most, if not all, of the null mutant map of the rosy locus contains structural information for the amino acid sequence of the XDH polypeptide. Consideration is given to the significance of the present results for the general problem of gene organization in higher eukaryotes.
Full Text
The Full Text of this article is available as a PDF (1.5 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Atwood K. C. Some aspects of the bobbed problem in Drosophila. Genetics. 1969;61(1 Suppl):319–327. [PubMed] [Google Scholar]
- Baker B. S., Carpenter A. T. Genetic analysis of sex chromosomal meiotic mutants in Drosophilia melanogaster. Genetics. 1972 Jun;71(2):255–286. doi: 10.1093/genetics/71.2.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chovnick A., Finnertty V., Schalet A., Duck P. Studies on genetic organization in higher organisms. I. Analysis of a complex gene in Drosophila melanogaster. Genetics. 1969 May;62(1):145–160. doi: 10.1093/genetics/62.1.145. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Georgiev G. P. On the structural organization of operon and the regulation of RNA synthesis in animal cells. J Theor Biol. 1969 Dec;25(3):473–490. doi: 10.1016/s0022-5193(69)80034-2. [DOI] [PubMed] [Google Scholar]
- Malling H. V., De Serres F. J. Genetic alterations at the molecular level in x-ray induced ad-3B mutants of Neurospora crassa. Radiat Res. 1973 Jan;53(1):77–87. [PubMed] [Google Scholar]
- McCarron M., Gelbart W., Chovnick A. Intracistronic mapping of electrophoretic sites in Drosophila melanogaster: fidelity of information transfer by gene conversion. Genetics. 1974 Feb;76(2):289–299. doi: 10.1093/genetics/76.2.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Plaut W., Nash D., Fanning T. Ordered replication of DNA in polytene chromosomes of Drosophila melanogaster. J Mol Biol. 1966 Mar;16(1):85–93. doi: 10.1016/s0022-2836(66)80264-4. [DOI] [PubMed] [Google Scholar]
- RITOSSA F. M., SPIEGELMAN S. LOCALIZATION OF DNA COMPLEMENTARY TO RIBOSOMAL RNA IN THE NUCLEOLUS ORGANIZER REGION OF DROSOPHILA MELANOGASTER. Proc Natl Acad Sci U S A. 1965 Apr;53:737–745. doi: 10.1073/pnas.53.4.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rasch E. M., Barr H. J., Rasch R. W. The DNA content of sperm of Drosophila melanogaster. Chromosoma. 1971;33(1):1–18. doi: 10.1007/BF00326379. [DOI] [PubMed] [Google Scholar]
- Rudkin G. T. The relative mutabilities of DNA in regions of the X chromosome of Drosophila melanogaster. Genetics. 1965 Sep;52(3):665–681. doi: 10.1093/genetics/52.3.665. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schalet A. Exchanges at the bobbed locus of Drosophila melanogaster. Genetics. 1969 Sep;63(1):133–153. doi: 10.1093/genetics/63.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]