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. 1993 Jul;65(1):492–498. doi: 10.1016/S0006-3495(93)81053-1

Effect of stress on the membrane capacitance of the auditory outer hair cell.

K H Iwasa 1
PMCID: PMC1225741  PMID: 8369452

Abstract

The membrane capacitance of the outer hair cell, which has unique membrane potential-dependent motility, was monitored during application of membrane tension. It was found that the membrane capacitance of the cell decreased when stress was applied to the membrane. This result is the opposite of stretching the lipid bilayer in the plasma membrane. It thus indicates the importance of some other capacitance component that decreases on stretching. It has been known that charge movement across the membrane can appear to be a nonlinear capacitance. If membrane stress at the resting potential restricts the movement of the charge associated with force generation, the nonlinear capacitance will decrease. Furthermore, less capacitance reduction by membrane stretching is expected when the membrane is already extended by the (hyperpolarizing) membrane potential. Indeed, it was found that at hyperpolarized potentials, the reduction of the membrane capacitance due to stretching is less. The capacitance change can be described by a two state model of a force-producing unit in which the free energy difference between the contracted and stretched states has both electrical and mechanical components. From the measured change in capacitance, the estimated difference in the membrane area of the unit between the two states is about 2 nm2.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashmore J. F. A fast motile response in guinea-pig outer hair cells: the cellular basis of the cochlear amplifier. J Physiol. 1987 Jul;388:323–347. doi: 10.1113/jphysiol.1987.sp016617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ashmore J. F. Forward and reverse transduction in the mammalian cochlea. Neurosci Res Suppl. 1990;12:S39–S50. doi: 10.1016/0921-8696(90)90007-p. [DOI] [PubMed] [Google Scholar]
  3. Bloom M., Evans E., Mouritsen O. G. Physical properties of the fluid lipid-bilayer component of cell membranes: a perspective. Q Rev Biophys. 1991 Aug;24(3):293–397. doi: 10.1017/s0033583500003735. [DOI] [PubMed] [Google Scholar]
  4. Brownell W. E., Bader C. R., Bertrand D., de Ribaupierre Y. Evoked mechanical responses of isolated cochlear outer hair cells. Science. 1985 Jan 11;227(4683):194–196. doi: 10.1126/science.3966153. [DOI] [PubMed] [Google Scholar]
  5. Dallos P., Evans B. N., Hallworth R. Nature of the motor element in electrokinetic shape changes of cochlear outer hair cells. Nature. 1991 Mar 14;350(6314):155–157. doi: 10.1038/350155a0. [DOI] [PubMed] [Google Scholar]
  6. Davis H. An active process in cochlear mechanics. Hear Res. 1983 Jan;9(1):79–90. doi: 10.1016/0378-5955(83)90136-3. [DOI] [PubMed] [Google Scholar]
  7. Gulley R. L., Reese T. S. Regional specialization of the hair cell plasmalemma in the organ of corti. Anat Rec. 1977 Sep;189(1):109–123. doi: 10.1002/ar.1091890108. [DOI] [PubMed] [Google Scholar]
  8. Holley M. C., Ashmore J. F. Spectrin, actin and the structure of the cortical lattice in mammalian cochlear outer hair cells. J Cell Sci. 1990 Jun;96(Pt 2):283–291. doi: 10.1242/jcs.96.2.283. [DOI] [PubMed] [Google Scholar]
  9. Iwasa K. H., Chadwick R. S. Elasticity and active force generation of cochlear outer hair cells. J Acoust Soc Am. 1992 Dec;92(6):3169–3173. doi: 10.1121/1.404194. [DOI] [PubMed] [Google Scholar]
  10. Iwasa K. H., Kachar B. Fast in vitro movement of outer hair cells in an external electric field: effect of digitonin, a membrane permeabilizing agent. Hear Res. 1989 Jul;40(3):247–254. doi: 10.1016/0378-5955(89)90165-2. [DOI] [PubMed] [Google Scholar]
  11. Iwasa K. H., Li M. X., Jia M., Kachar B. Stretch sensitivity of the lateral wall of the auditory outer hair cell from the guinea pig. Neurosci Lett. 1991 Dec 9;133(2):171–174. doi: 10.1016/0304-3940(91)90562-8. [DOI] [PubMed] [Google Scholar]
  12. Kachar B., Brownell W. E., Altschuler R., Fex J. Electrokinetic shape changes of cochlear outer hair cells. Nature. 1986 Jul 24;322(6077):365–368. doi: 10.1038/322365a0. [DOI] [PubMed] [Google Scholar]
  13. Kalinec F., Holley M. C., Iwasa K. H., Lim D. J., Kachar B. A membrane-based force generation mechanism in auditory sensory cells. Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8671–8675. doi: 10.1073/pnas.89.18.8671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mountain D. C., Hubbard A. E. Rapid force production in the cochlea. Hear Res. 1989 Nov;42(2-3):195–202. doi: 10.1016/0378-5955(89)90144-5. [DOI] [PubMed] [Google Scholar]
  15. Sachs F., Lecar H. Stochastic models for mechanical transduction. Biophys J. 1991 May;59(5):1143–1145. doi: 10.1016/S0006-3495(91)82329-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Santos-Sacchi J., Dilger J. P. Whole cell currents and mechanical responses of isolated outer hair cells. Hear Res. 1988 Sep 15;35(2-3):143–150. doi: 10.1016/0378-5955(88)90113-x. [DOI] [PubMed] [Google Scholar]
  17. Santos-Sacchi J. Reversible inhibition of voltage-dependent outer hair cell motility and capacitance. J Neurosci. 1991 Oct;11(10):3096–3110. doi: 10.1523/JNEUROSCI.11-10-03096.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sokabe M., Sachs F., Jing Z. Q. Quantitative video microscopy of patch clamped membranes stress, strain, capacitance, and stretch channel activation. Biophys J. 1991 Mar;59(3):722–728. doi: 10.1016/S0006-3495(91)82285-8. [DOI] [PMC free article] [PubMed] [Google Scholar]

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