Abstract
The effects of ovariectomy at day 12 of pregnancy on the proliferative activity of the uterine epithelium, metrial gland and uterine muscle in the rat were studied by autoradiography in animals killed one hour after a single injection of tritiated thymidine. The results were amplified by histological and histochemical studies. One day after operation epithelial proliferation was impaired in the ovarectomized animals. From 16 days onwards, however, proliferative activity was at a lower level in controls than in the ovariectomized animals. Three days after ovariectomy uptake of tritiated thymidine by metrial gland cells was less than in the controls. The metrial gland became smaller after ovariectomy and showed histological and histochemical changes from the normal pattern. The increased thickness of the uterine muscle in the ovariectomized animals from the 16 day stage onwards was not associated with any significant change in the labelling index of the muscle cells. The results are discussed in relation to the changes in endocrine environment which may occur after ovariectomy.
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- BLOCH S. DIE GLANDULA MYOMETRALIS IM UTERUS DER MAUS. Acta Anat (Basel) 1964;56:103–119. [PubMed] [Google Scholar]
- Bulmer D. Further studies on the granulated metrial gland cells of the pregnant rat. J Anat. 1968 Nov;103(Pt 3):479–489. [PMC free article] [PubMed] [Google Scholar]
- Bulmer D., Peel S. An autoradiographic study of cellular proliferation in the uterus and placenta of the pregnant rat. J Anat. 1974 Apr;117(Pt 2):433–441. [PMC free article] [PubMed] [Google Scholar]
- CANIVENC R. L'activité endocrine du placenta de la rate. Arch Anat Histol Embryol. 1951;34(1-8):105–117. [PubMed] [Google Scholar]
- CANIVENC R., MAYER G. Placenta et équilibre hormonal gravidique chez la rate; activité des stéroïdes sexuels sur la glande mammaire et le vagin de la rate gravide castrée. C R Seances Soc Biol Fil. 1951 Nov;145(21-22):1692–1695. [PubMed] [Google Scholar]
- Dallenbach-Hellweg G., Battista J. V., Dallenbach F. D. Immunohistological and histochemical localization of relaxin in the metrial gland of the pregnant rat. Am J Anat. 1965 Nov;117(3):433–450. doi: 10.1002/aja.1001170308. [DOI] [PubMed] [Google Scholar]
- Larkin L. H. Bioassay of rat metrial gland extracts for relaxin using the mouse interpubic ligament technique. Endocrinology. 1974 Feb;94(2):567–570. doi: 10.1210/endo-94-2-567. [DOI] [PubMed] [Google Scholar]
- Larkin L. H., Schultz R. L. Histochemical and autoradiographic studies of the formation of the metrial gland in the pregnant rat. Am J Anat. 1968 May;122(3):607–619. doi: 10.1002/aja.1001220311. [DOI] [PubMed] [Google Scholar]
- MAYER G., CANIVENC R. Autogreffes de placenta chez la rate. C R Seances Soc Biol Fil. 1950 Mar;144(5-6):410–412. [PubMed] [Google Scholar]
- MAYER G., CANIVENC R. Placenta et équilibre hormonal gravidique chez la rate; activité du placenta en l'absence d'ovaires; réaction de la glande mammaire et du vagin. C R Seances Soc Biol Fil. 1951 Nov;145(21-22):1688–1692. [PubMed] [Google Scholar]
- Peel S., Bulmer D. A study of proliferative activity of the uterine epithelium of the pregnant rat in relation to the morphogenesis of the "new" lumen. J Reprod Fertil. 1975 Jan;42(1):189–193. doi: 10.1530/jrf.0.0420189. [DOI] [PubMed] [Google Scholar]
- Pepe G. J., Rothchild I. Serum progesterone levels in ovariectomized rats injected with progesterone and estrone: relation to pregnancy maintenance and growth of decidual tissue. Endocrinology. 1973 Nov;93(5):1193–1199. doi: 10.1210/endo-93-5-1193. [DOI] [PubMed] [Google Scholar]
- RAY E. W., AVERILL S. C., LYONS W. R., JOHNSON R. E. Rat placental hormonal activities corresponding to those of pituitary mammotropin. Endocrinology. 1955 Apr;56(4):359–373. doi: 10.1210/endo-56-4-359. [DOI] [PubMed] [Google Scholar]