Abstract
Small pieces of tissue obtained from apparently normal areas of four surgically removed adult human kidneys were used in the present study. The results obtained by immuno-fluorescence and immuno-electron microscopical techniques show that Tamm-Horsfall glycoprotein (THP) is present in the thick ascending limbs of the loops of Henle and the distal convoluted tubules. Within the cells concerned, the protein is associated with the luminal, lateral as well as basal, plasma membranes and their infoldings. The cells of the macula densa are completely negative as are those of proximal convoluted tubules, glomeruli and collecting ducts. The possible significance of these findings in relation to the process of urine dilution in the nephron is discussed.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Hoyer J. R., Seiler M. W. Pathophysiology of Tamm-Horsfall protein. Kidney Int. 1979 Sep;16(3):279–289. doi: 10.1038/ki.1979.130. [DOI] [PubMed] [Google Scholar]
- Hoyer J. R., Sisson S. P., Vernier R. L. Tamm-Horsfall glycoprotein: ultrastructural immunoperoxidase localization in rat kidney. Lab Invest. 1979 Aug;41(2):168–173. [PubMed] [Google Scholar]
- McKenzie J. K., McQueen E. G. Immunofluorescent localization of Tamm-Horsfall mucoprotein in human kidney. J Clin Pathol. 1969 May;22(3):334–339. doi: 10.1136/jcp.22.3.334. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McLean I. W., Nakane P. K. Periodate-lysine-paraformaldehyde fixative. A new fixation for immunoelectron microscopy. J Histochem Cytochem. 1974 Dec;22(12):1077–1083. doi: 10.1177/22.12.1077. [DOI] [PubMed] [Google Scholar]
- Pollak V. E., Arbel C. The distribution of Tamm Horsfall mucoprotein (uromucoid) in the human nephron. Nephron. 1969;6(6):667–672. doi: 10.1159/000179766. [DOI] [PubMed] [Google Scholar]
- Resnick J. S., Sisson S., Vernier R. L. Tamm-Horsfall protein. Abnormal localization in renal disease. Lab Invest. 1978 May;38(5):550–555. [PubMed] [Google Scholar]
- SOBER H. A., PETERSON E. A. Protein chromatography on ion exchange cellulose. Fed Proc. 1958 Dec;17(4):1116–1126. [PubMed] [Google Scholar]
- Schenk E. A., Schwartz R. H., Lewis R. A. Tamm-Horsfall mucoprotein. I. Localization in the kidney. Lab Invest. 1971 Jul;25(1):92–95. [PubMed] [Google Scholar]
- Sikri K. L., Foster C. L. Light and electron microscopical observations on the macula densa of the Syrian hamster kidney. J Anat. 1981 Jan;132(Pt 1):57–69. [PMC free article] [PubMed] [Google Scholar]
- Sisson S. P., Vernier R. L. Methods for immunoelectron microscopy: Localization of antigens in rat kidney. J Histochem Cytochem. 1980 May;28(5):441–452. doi: 10.1177/28.5.6991591. [DOI] [PubMed] [Google Scholar]
- TAMM I., HORSFALL F. L., Jr A mucoprotein derived from human urine which reacts with influenza, mumps, and Newcastle disease viruses. J Exp Med. 1952 Jan;95(1):71–97. doi: 10.1084/jem.95.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TAMM I., HORSFALL F. L., Jr Characterization and separation of an inhibitor of viral hemagglutination present in urine. Proc Soc Exp Biol Med. 1950 May;74(1):106–108. [PubMed] [Google Scholar]
- Zager R. A., Cotran R. S., Hoyer J. R. Pathologic localization of Tamm-Horsfall protein in interstitial deposits in renal disease. Lab Invest. 1978 Jan;38(1):52–57. [PubMed] [Google Scholar]