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. 1996 Oct;71(4):1796–1805. doi: 10.1016/S0006-3495(96)79380-3

Determination of molecular order in supported lipid membranes by internal reflection Fourier transform infrared spectroscopy.

M J Citra 1, P H Axelsen 1
PMCID: PMC1233648  PMID: 8889156

Abstract

When polarized internal reflection infrared spectroscopy is used to determine molecular order in supported lipid membranes, the results are critically dependent on the accuracy of assumptions made about the evanescent electric field amplitudes in the membrane. In this work, we examine several expressions used for calculating evanescent electric field amplitudes in supported lipid monolayers and bilayers, and test their validity by measuring the infrared dichroism of poly-gamma-benzyl-L-glutamate and poly-beta-benzyl-L-aspartate under conditions in which their molecular order is known. Our results indicate that treating such systems as a simple single interface between two semi-infinite bulk phases is more accurate than the commonly employed thin-film approximation. This implies that earlier conclusions about molecular order in supported lipid membranes may require substantial revision.

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Selected References

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  1. Axelsen P. H., Kaufman B. K., McElhaney R. N., Lewis R. N. The infrared dichroism of transmembrane helical polypeptides. Biophys J. 1995 Dec;69(6):2770–2781. doi: 10.1016/S0006-3495(95)80150-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BRADBURY E. M., BROWN L., DOWNIE A. R., ELLIOTT A., FRASER R. D., HANBY W. E. The structure of the omegaform of poly-Beta-benzyl-L-aspartate. J Mol Biol. 1962 Aug;5:230–247. doi: 10.1016/s0022-2836(62)80086-2. [DOI] [PubMed] [Google Scholar]
  3. Bradbury E. M., Carpenter B. G., Stephens R. M. Conformational studies of polymers and copolymers of L-aspartate ester. II. Infrared studies and the factors involved in the formation of the omega-helix. Biopolymers. 1968;6(7):905–915. doi: 10.1002/bip.1968.360060702. [DOI] [PubMed] [Google Scholar]
  4. Brauner J. W., Mendelsohn R., Prendergast F. G. Attenuated total reflectance Fourier transform infrared studies of the interaction of melittin, two fragments of melittin, and delta-hemolysin with phosphatidylcholines. Biochemistry. 1987 Dec 15;26(25):8151–8158. doi: 10.1021/bi00399a020. [DOI] [PubMed] [Google Scholar]
  5. Frey S., Tamm L. K. Orientation of melittin in phospholipid bilayers. A polarized attenuated total reflection infrared study. Biophys J. 1991 Oct;60(4):922–930. doi: 10.1016/S0006-3495(91)82126-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Giancotti V., Quadrifoglio F., Crescenzi V. Helix sense of poly- -benzyl-L-aspartate. J Am Chem Soc. 1972 Jan 12;94(1):297–298. doi: 10.1021/ja00756a063. [DOI] [PubMed] [Google Scholar]
  7. Kalb E., Frey S., Tamm L. K. Formation of supported planar bilayers by fusion of vesicles to supported phospholipid monolayers. Biochim Biophys Acta. 1992 Jan 31;1103(2):307–316. doi: 10.1016/0005-2736(92)90101-q. [DOI] [PubMed] [Google Scholar]
  8. Lüneberg J., Martin I., Nüssler F., Ruysschaert J. M., Herrmann A. Structure and topology of the influenza virus fusion peptide in lipid bilayers. J Biol Chem. 1995 Nov 17;270(46):27606–27614. doi: 10.1074/jbc.270.46.27606. [DOI] [PubMed] [Google Scholar]
  9. Martin I., Schaal H., Scheid A., Ruysschaert J. M. Lipid membrane fusion induced by the human immunodeficiency virus type 1 gp41 N-terminal extremity is determined by its orientation in the lipid bilayer. J Virol. 1996 Jan;70(1):298–304. doi: 10.1128/jvi.70.1.298-304.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. PERUTZ M. F. New x-ray evidence on the configuration of polypeptide chains. Nature. 1951 Jun 30;167(4261):1053–1054. doi: 10.1038/1671053a0. [DOI] [PubMed] [Google Scholar]
  11. Smith S. O., Jonas R., Braiman M., Bormann B. J. Structure and orientation of the transmembrane domain of glycophorin A in lipid bilayers. Biochemistry. 1994 May 24;33(20):6334–6341. doi: 10.1021/bi00186a037. [DOI] [PubMed] [Google Scholar]
  12. Tsujita Y. Reversal in helix sense of copoly(beta-alkyl-L-aspartate-beta-benzyl-L-aspartate). Biophys Chem. 1988 Aug;31(1-2):11–20. doi: 10.1016/0301-4622(88)80003-6. [DOI] [PubMed] [Google Scholar]
  13. Vogel H., Jähnig F., Hoffmann V., Stümpel J. The orientation of melittin in lipid membranes. A polarized infrared spectroscopy study. Biochim Biophys Acta. 1983 Sep 7;733(2):201–209. doi: 10.1016/0005-2736(83)90523-0. [DOI] [PubMed] [Google Scholar]

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